Influence of Saponins on Lipid Bilayers

皂苷对脂质双层的影响

• 批准号: 315438106
• 负责人: Professor Dr. Thomas Hellweg
• 金额: --
• 依托单位: Arbeitsgruppe Physikalische und Biophysikalische Chemie (PC III)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/315438106?language=en
• 关键词: Influence; Saponins; Lipid; Bilayers

Saponins have a multitude of different effects in living systems. In most cases these effects can be related to the interaction of saponins with the cell membranes. For this reason saponins are studied intensely since many decades. However, despite the pharmacological importance and despite of the numerous studies on saponins information about the local interaction between lipids, cholestrol and saponins is scarce. The present project we will study model membranes (vesicle) made of different lipids and saponins in presence of different amounts of cholesterol by means of neutron spin-echo spectroscopy. The quasielastic experiments will be complemented by small angle and wide angle scattering aiming at establishing relations between local structure and bending elasticity of the bilayers. In addition photon correlation spectroscopy and flickering spectroscopy will be used to scrutinize the membrane motion on larger length and time scales. We are aiming at establishing a relationschip between saponin induced changes in membrane properties to the known biological effects of saponins.

皂苷在生命系统中有多种不同的作用。在大多数情况下，这些作用可能与皂苷与细胞膜的相互作用有关。正因为如此，近几十年来，人们对皂苷的研究非常深入。然而，尽管对皂苷有重要的药理作用和大量的研究，但关于脂类、胆固醇和皂苷之间的局部相互作用的信息很少。在本项目中，我们将用中子自旋回波谱的方法研究不同脂类和皂苷在不同胆固醇存在下制成的模型膜(囊泡)。准弹性实验将补充小角度和广角散射，目的是建立双层的局部结构和弯曲弹性之间的关系。此外，还将使用光子相关光谱和闪烁光谱在更大的长度和时间尺度上仔细观察膜的运动。我们的目标是建立皂苷引起的膜特性变化与皂苷已知生物学效应之间的关系。