Influence of Saponins on Lipid Bilayers

皂苷对脂质双层的影响

• 批准号: 315438106
• 负责人: Professor Dr. Thomas Hellweg
• 金额: --
• 依托单位: Arbeitsgruppe Physikalische und Biophysikalische Chemie (PC III)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/315438106?language=en
• 关键词: Influence; Saponins; Lipid; Bilayers

Saponins have a multitude of different effects in living systems. In most cases these effects can be related to the interaction of saponins with the cell membranes. For this reason saponins are studied intensely since many decades. However, despite the pharmacological importance and despite of the numerous studies on saponins information about the local interaction between lipids, cholestrol and saponins is scarce. The present project we will study model membranes (vesicle) made of different lipids and saponins in presence of different amounts of cholesterol by means of neutron spin-echo spectroscopy. The quasielastic experiments will be complemented by small angle and wide angle scattering aiming at establishing relations between local structure and bending elasticity of the bilayers. In addition photon correlation spectroscopy and flickering spectroscopy will be used to scrutinize the membrane motion on larger length and time scales. We are aiming at establishing a relationschip between saponin induced changes in membrane properties to the known biological effects of saponins.

皂苷在生命系统中具有多种不同的作用。在大多数情况下，这些作用可能与皂苷与细胞膜的相互作用有关。由于这个原因，几十年来一直在深入研究皂苷。然而，尽管药理学的重要性，尽管有大量的研究皂苷信息之间的局部相互作用的脂质，胆固醇和皂苷是稀缺的。本计画将利用中子自旋回波光谱法，研究由不同脂质及皂甙所组成的模型膜（囊泡），以及在不同胆固醇含量下的膜结构。准弹性实验将补充小角和广角散射，旨在建立局部结构和弯曲弹性的双层之间的关系。此外，光子相关光谱学和闪烁光谱学将用于在更大的长度和时间尺度上仔细检查膜运动。我们的目标是建立皂苷诱导的膜特性变化与皂苷已知的生物学效应之间的关系。