Industrial saponins

工业皂苷

• 批准号: BB/M028712/1
• 负责人: Anne Osbourn
• 金额: $ 58.68万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FM028712%2F1
• 关键词: Industrial; saponins

The aim of this project is to bring saponin production into the domain of industrial biotechnology to make this diverse group of biosurfactants available for commercial exploitation at the required scale, structure specificity & cost, in the first instance for home & personal care (HPC) use. We have already demonstrated that saponins can synergise with conventional surfactants to reduce the total surfactant level required for cleaning performance in laundry detergents, and that such mixtures are particularly effective at low temperatures. Saponins are plant-derived triterpenoidal or steroidal surfactants. Saponins currently have limited commercial availability, being sourced from natural plant materials. This supply is deficient in scale, is of limited structure specificity and is too expensive for Unilever's intended use in laundry detergents. Unilever's concentrated detergents business alone would require 100 tonnes pa of saponin at 0.1% level and 20% product range penetration. Leading saponin supplier Desert King produces only 20 tonnes active saponin pa from natural extracts. Furthermore, we have demonstrated that certain saponin structures are significantly more effective than others. The current sources of saponins are natural extracts and comprise a heterogeneous mixture of saponin structures. Our IB innovation will enable considerable enrichment for the most effective saponin structures. Current commercial saponins are too expensive at $35/kg for crude extracts for use in anything other than premium products. We expect IB to be a more cost effective route to saponin supply.

该项目的目的是将皂苷生产纳入工业生物技术领域，使这种多样化的生物表面活性剂可用于所需规模，结构特异性和成本的商业开发，首先用于家庭和个人护理（HPC）用途。我们已经证明，皂苷可以与常规表面活性剂协同作用，以降低洗衣洗涤剂中清洁性能所需的总表面活性剂水平，并且这种混合物在低温下特别有效。皂苷是植物来源的三萜类或甾体表面活性剂。皂苷目前具有有限的商业可用性，其来源于天然植物材料。这种供应在规模上是不足的，具有有限的结构特异性，并且对于联合利华在洗衣洗涤剂中的预期用途来说太昂贵。仅联合利华的浓缩洗涤剂业务就需要每年100吨0.1%水平的皂苷和20%的产品范围渗透率。领先的皂苷供应商沙漠国王生产只有20吨活性皂苷pa从天然提取物。此外，我们已经证明某些皂苷结构比其他结构显著更有效。皂苷的当前来源是天然提取物，并且包含皂苷结构的异质混合物。我们的IB创新将使最有效的皂苷结构得到相当大的富集。目前的商业皂苷对于用于除了优质产品之外的任何东西的粗提取物来说都太昂贵了，为35美元/kg。我们希望IB是一种更具成本效益的皂苷供应途径。

项目成果

Plant metabolic clusters - from genetics to genomics.

• DOI: 10.1111/nph.13981
• 发表时间: 2016-08
• 期刊: The New phytologist
• 作者: Nützmann HW;Huang A;Osbourn A
• 通讯作者: Osbourn A

Computational genomic identification and functional reconstitution of plant natural product biosynthetic pathways.

• DOI: 10.1039/c6np00035e
• 发表时间: 2016-08-27
• 期刊: Natural product reports
• 影响因子: 11.9
• 作者: Medema MH;Osbourn A
• 通讯作者: Osbourn A

Minimum Information about a Biosynthetic Gene cluster.

• DOI: 10.1038/nchembio.1890
• 发表时间: 2015-09
• 期刊: Nature chemical biology
• 影响因子: 14.8
• 作者: Medema MH;Kottmann R;Yilmaz P;Cummings M;Biggins JB;Blin K;de Bruijn I;Chooi YH;Claesen J;Coates RC;Cruz-Morales P;Duddela S;Düsterhus S;Edwards DJ;Fewer DP;Garg N;Geiger C;Gomez-Escribano JP;Greule A;Hadjithomas M;Haines AS;Helfrich EJ;Hillwig ML;Ishida K;Jones AC;Jones CS;Jungmann K;Kegler C;Kim HU;Kötter P;Krug D;Masschelein J;Melnik AV;Mantovani SM;Monroe EA;Moore M;Moss N;Nützmann HW;Pan G;Pati A;Petras D;Reen FJ;Rosconi F;Rui Z;Tian Z;Tobias NJ;Tsunematsu Y;Wiemann P;Wyckoff E;Yan X;Yim G;Yu F;Xie Y;Aigle B;Apel AK;Balibar CJ;Balskus EP;Barona-Gómez F;Bechthold A;Bode HB;Borriss R;Brady SF;Brakhage AA;Caffrey P;Cheng YQ;Clardy J;Cox RJ;De Mot R;Donadio S;Donia MS;van der Donk WA;Dorrestein PC;Doyle S;Driessen AJ;Ehling-Schulz M;Entian KD;Fischbach MA;Gerwick L;Gerwick WH;Gross H;Gust B;Hertweck C;Höfte M;Jensen SE;Ju J;Katz L;Kaysser L;Klassen JL;Keller NP;Kormanec J;Kuipers OP;Kuzuyama T;Kyrpides NC;Kwon HJ;Lautru S;Lavigne R;Lee CY;Linquan B;Liu X;Liu W;Luzhetskyy A;Mahmud T;Mast Y;Méndez C;Metsä-Ketelä M;Micklefield J;Mitchell DA;Moore BS;Moreira LM;Müller R;Neilan BA;Nett M;Nielsen J;O'Gara F;Oikawa H;Osbourn A;Osburne MS;Ostash B;Payne SM;Pernodet JL;Petricek M;Piel J;Ploux O;Raaijmakers JM;Salas JA;Schmitt EK;Scott B;Seipke RF;Shen B;Sherman DH;Sivonen K;Smanski MJ;Sosio M;Stegmann E;Süssmuth RD;Tahlan K;Thomas CM;Tang Y;Truman AW;Viaud M;Walton JD;Walsh CT;Weber T;van Wezel GP;Wilkinson B;Willey JM;Wohlleben W;Wright GD;Ziemert N;Zhang C;Zotchev SB;Breitling R;Takano E;Glöckner FO
• 通讯作者: Glöckner FO

Harnessing plant metabolic diversity.

• DOI: 10.1016/j.cbpa.2017.04.015
• 发表时间: 2017-10
• 期刊: Current opinion in chemical biology
• 影响因子: 7.8
• 作者: Owen C;Patron NJ;Huang A;Osbourn A
• 通讯作者: Osbourn A

A translational synthetic biology platform for rapid access to gram-scale quantities of novel drug-like molecules.

• DOI: 10.1016/j.ymben.2017.06.012
• 发表时间: 2017-07
• 期刊: Metabolic engineering
• 影响因子: 8.4
• 作者: Reed J;Stephenson MJ;Miettinen K;Brouwer B;Leveau A;Brett P;Goss RJM;Goossens A;O'Connell MA;Osbourn A
• 通讯作者: Osbourn A