Development of a three-dimensional model of structural and functional changes during skeletal muscle growth: Experiment, simulation and validation

骨骼肌生长过程中结构和功能变化的三维模型的开发：实验、模拟和验证

• 批准号: 316485047
• 负责人: Professor Dr.-Ing. Markus Böl
• 金额: --
• 依托单位: Abteilung für Bewegungs- und Trainingswissenschaft
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/316485047?language=en
• 关键词: Development; three; dimensional; model; structural

During growth muscle tissue is subjected to extensive structural and mechanical changes. Thereby, the increase of muscle force is related to the adaptation of the three-dimensional muscle geometry and the material behaviour of the muscle. The whole growth process happens while retaining the muscle function. The essential condition for the understanding and the modelling of growth is the acquisition of the structural and mechanical changes. Aim of the project is the development and validation of a growth model for skeletal muscles. This aim is ensured by two interacting steps, that are characterised by a tight complexity between experiment and modelling. The first project phase deals with the development of a growth model for the simple structured rabbit m. soleus. Rooted on experiments, we identify at 10 different animal ages (between the 4th and the 20th week of life) specific muscle parameters. This experimental work comprises the determination of the active muscle characteristics, the passive tissue behaviour, the muscle deformation during contraction, the three-dimensional muscle architecture, as well as the immunohistologic analysis of the muscle tissue. Based on these experimental results, growth kinetics for various state variables (e.g. pennation angle, tissue stiffness, fibre typ) will be identified, which will be used in a subsequent step for the development of the growth models (for muscle, aponeuroses, and tendon tissue). The model validation occurs on experimental force and deformation data that have been imposed from dynamic muscle contraction experiments for all muscle ages. In a second project phase the growth model will be verified on the much more complex m. plantaris. This multi-pennate muscle is characterised by a complex fibre architecture combined with inner tendon sheaths as well as an inhomogeneous fibre type distribution. A successful development and validation of the growth models as planed in this project would accelerate the knowledge concerning the growth behaviour of skeletal muscles in a distinct way. The knowledge of structural changes and contractile characteristics is a crucial requirement for a deeper understanding of muscle shape changes and the differentiation during growth. An adequate growth model allows the studying of state variables inside the muscle (e.g. inner pressure, stresses, fibre orientation) as well as their change during growth. This could lead to a better understanding of biomechanical relations. Furthermore, the successful development of growth models could be a basis to answer open evolutionary-biological questions like the three-dimensional arrangement (assembly of muscles in muscle packages) and the growth of extremities.

在生长过程中，组织会经历广泛的结构和机械变化。因此，肌肉力的增加与三维肌肉几何形状的适应和肌肉的物质行为有关。整个生长过程在保持肌肉功能时发生。理解和建模生长的基本条件是对结构和机械变化的获取。该项目的目的是开发和验证骨骼肌的增长模型。通过两个相互作用的步骤确保了这一目标，这些步骤的特征是实验和建模之间的复杂性紧密。第一个项目阶段涉及简单结构兔M的增长模型的发展。比目鱼。植根于实验，我们在10个不同的动物年龄（生命的第4周至第20周之间）识别特定的肌肉参数。这项实验性工作包括确定活性肌肉特征，被动组织行为，收缩过程中的肌肉变形，三维肌肉结构以及对肌肉组织的免疫组织学分析。基于这些实验结果，将鉴定出各种状态变量的生长动力学（例如，磨损角度，组织刚度，纤维典型），将在随后的发展步骤中使用，用于开发生长模型（用于肌肉，蛋白神经脂和肌腱组织）。该模型验证发生在所有肌肉年龄的动态肌肉收缩实验中施加的实验力和变形数据上。在第二个项目阶段，将在更复杂的M上验证增长模型。 Plantaris。这种多型肌肉的特征是复杂的纤维结构，结合了内部肌腱鞘以及不均匀的纤维类型分布。该项目计划的成功开发和验证将加速有关骨骼肌肉生长行为的知识。对结构变化和收缩特征的了解是对肌肉形状变化和生长过程中的分化的更深入了解的关键要求。适当的增长模型允许研究肌肉内部的状态变量（例如内部压力，压力，纤维取向）及其在生长过程中的变化。这可能会导致对生物力学关系的更好理解。此外，增长模型的成功发展可能是回答开放的进化生物学问题的基础，例如三维安排（肌肉包装中的肌肉组装）和四肢的生长。