From muscle fibre to tissue - experiment, modelling and simulation at micro, meso and makro scale

从肌肉纤维到组织 - 微观、中观和宏观尺度的实验、建模和模拟

基本信息

批准号：
258667461
负责人：
Professor Dr.-Ing. Markus Böl
金额：
--
依托单位：
Institut für Mikrotechnik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2014
资助国家：
德国
起止时间：
2013-12-31 至 2022-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/258667461?language=en
关键词：
muscle fibre tissue experiment modelling

项目摘要

The present project deals with the analysis of complex deformation states of skeletal muscle tissue whose results will be integrated in a numerical multi-scale modelling approach. The essential aim within the project is the determination of the mechanical behaviour of the extracellular matrix (ECM) and consequently the prediction of changes based on quantitative and/or structural changes of the ECM. This knowledge, however, is of high medical as well as socioeconomical importance but rooted on previous analytical methods it could not be obtained. In order to advance research on this field, the present project combines two areas of expertise: microsystems technology and solid mechanics. This unique combination presents a completely new and innovative approach and enables the development of new methods in order to analyse skeletal muscle tissue from the experimental as well as numerical point of view.Therefore, this project provides extensive experimental analyses on different size scales. In a first step we apply at micro scale (10-90 µm) axial tension as well as axial/transversal compression experiments on single muscle fibres and muscle fibres segments, respectively. Further, at meso scale (0,1-1 mm), we focus on compression and shear-compression tests applied on cubes excised from muscle fascicles. Finally, cubes resected from muscle tissue will be tested at macro scale (1-24 mm).In order to perform such experiments special setups at micro and meso scale need to be developed. Their main components will be micro technological parallel grippers. In doing so, single muscle fibres or cubes resected from fascicles by means of ultra-short-pulse laser can be loaded and forces, displacements and deformations can be measured. Experiments at macro scale will be accomplished using well-known experimental methods.Based on these multi-scale experiments and by means of the inverse finite element method we establish a three-step identification process that allows to determinate the mechanical behaviour of ECM. Finally, rooted on these findings a multi-scale modelling approach and its validation is scheduled.

本项目介绍了骨骼肌组织的复杂变形状态的分析，其结果将集成在数值多规模建模方法中。该项目的基本目的是确定细胞外基质（ECM）的机械行为，因此根据ECM的定量和/或结构变化来预测变化。但是，这种知识具有很高的医学和社会经济重要性，但植根于先前的分析方法。为了进步对该领域的研究，本项目结合了两个专家领域：微系统技术和固体机制。这种独特的组合提出了一种全新的创新方法，并可以开发新方法，以便从实验和数值的角度分析骨骼肌组织。因此，该项目在不同尺寸尺度上提供了广泛的实验分析。在第一步中，我们分别在单肌纤维和肌肉纤维段上以微尺度（10-90 µm）的轴向张力以及轴向/横向压缩实验进行应用。此外，在Meso量表（0,1-1毫米）中，我们专注于对肌肉束的极好的立方体应用的压缩和剪切压缩测试。最后，从肌肉组织中矫正的立方体将以宏观量表（1-24 mm）进行测试。为了在微观上进行此类实验，需要开发这种实验，并且需要开发中等量表。它们的主要组件将是微型技术平行抓手。这样，可以通过超短脉冲激光器从束缚中切除的单肌纤维或立方体可以加载，并可以测量力，位移和变形。将使用众所周知的实验方法来完成宏观量表的实验。基于这些多尺度实验，并通过反向结局元素方法来建立一个三步识别过程，该过程允许确定ECM的机械行为。最后，植根于这些发现的多尺度建模方法及其验证。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Micro-Gripper: A new concept for a monolithic single-cell manipulation device

DOI：
10.1016/j.sna.2015.10.024
发表时间：
2015-12-01
期刊：
SENSORS AND ACTUATORS A-PHYSICAL
影响因子：
4.6
作者：
Garces-Schroeder, Mayra;Leester-Schaedel, Monika;Dietzel, Andreas
通讯作者：
Dietzel, Andreas

Shape Memory Alloy Actuators for Silicon Microgrippers

DOI：
10.1109/jmems.2019.2936288
发表时间：
2019-10-01
期刊：
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
影响因子：
2.7
作者：
Garces-Schroeder, Mayra;Zimmermann, Tom;Dietzel, Andreas
通讯作者：
Dietzel, Andreas

Characterization of skeletal muscle passive mechanical properties by novel micro-force sensor and tissue micro-dissection by femtosecond laser ablation

DOI：
10.1016/j.mee.2018.01.024
发表时间：
2018-05
期刊：
Microelectronic Engineering
影响因子：
2.3
作者：
Mayra Garcés-Schröder;D. Metz;Lars Hecht;Rahul Iyer;M. Leester-Schädel;M. Böl;A. Dietzel
通讯作者：
Mayra Garcés-Schröder;D. Metz;Lars Hecht;Rahul Iyer;M. Leester-Schädel;M. Böl;A. Dietzel

Direct measurement of the direction-dependent mechanical behaviour of skeletal muscle extracellular matrix.

直接测量骨骼肌细胞外基质的方向依赖性机械行为