発電機能を持つ歯周病細菌におけると鉄イオン排出機構に関する研究

牙龈卟啉单胞菌（PG）是发现于牙齿表面的牙菌斑生物膜的典型成员。PG是慢性口腔疾病和全身炎症性疾病（如心血管、关节炎和神经退行性疾病）相关的主要病原体。本研究旨在确定EET耦合铁输出在PG中导致炎症和腐蚀的关键机制。PG能吸收可溶性Fe2+，以氧化态Fe3+的形式储存在细胞内，在缺铁时利用。然而，虽然PG对避免过量铁中毒至关重要，但PG铁的稳态和输出机制尚不清楚。本研究考察并比较了PG利用不同氨基底物的胞外电子传递（EET）能力。与其他底物相比，牙龈卟啉卟啉的库仑效率（CE）为2.8%，显著提高了组氨酸代谢电流，比之前报道的牙龈卟啉卟啉的葡萄糖氧化效率（0.003%）高出约1000倍。在本研究中，对牙龈卟啉卟啉对铁还原的重要鉴定进行了测试，并鉴定出相同的鉴定结果，提示了EET与牙龈卟啉卟啉对铁还原的可能机制。铁对病原菌生长的影响还表现在电子传递能力上。我们的研究结果表明，氨基酸，特别是组氨酸，其目前的生产速度比葡萄糖高8倍，可以作为无糖分解病原体牙龈卟啉卟啉的碳源。

Porphyromonas gingivalis (PG) is a typical member of the dental plaque biofilm found on the tooth surface. PG is a keystone pathogen associated with chronic oral diseases and systemic inflammatory disorders such as cardiovascular, arthritic, and neurodegenerative diseases. This research aims to identify the critical mechanisms of EET coupled iron export in PG leading to inflammation and corrosion. PG can take up soluble Fe2+ and store it in oxidized Fe3+ form intracellularly to utilize in times of iron limitation. However, whilst essential to avoid toxicity due to excess iron, PG iron homeostasis and the export mechanism is not well understood. This study investigated and compared the extracellular elecctron transport (EET) capabilities of PG using various amino substrates. With a coulombic efficiency (CE) of 2.8%, P. gingivalis considerably increased current with histidine metabolism compared to other substrates, which is around 1000 times higher than the CE that P. gingivalis was previously reported to have for glucose oxidation (0.003%). During this research, important identification of Iron reduction by P. gingivalis was tested and identified the same, indicating the possible mechanism of EET coupled with Iron reduction by P. gingivalis. And iron has showed the impact on the growth of pathogen reflected in the electron transfer capability as well. Our findings demonstrate that amino acids, particularly histidine, which has an eight times greater current production rate than glucose, can serve as carbon sources for the asaccharolytic pathogen P. gingivalis.