Signal-amplified two-way detection of multiple viruses using chromogen-embedded nanocarriers

使用显色剂嵌入纳米载体对多种病毒进行信号放大双向检测

• 批准号: 20J22358
• 负责人: コリス インドラ メンディ
• 金额: $ 1.79万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-24 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J22358/
• 关键词: Virus detection; Signal Amplification; Biosensor; Aptamer; Electrochemical; Colorimetric; Nanozyme Activity; Nanomaterial; Virus Detection; Signal Enrichment; colorimetric; Polymer nanomaterial; Derivative spectroscopy; Analytical Chemistry /

In the last year of this fellowship, the aim focused on developing highly sensitive biosensor for virus detection. Compared to previous year, the developed chromogenic nanocarrier using encapsulation is not suitable for electrochemical sensing system. Then, we adjusted the method and chelating the signal molecules carried within the aptamer network on the electrode to amplify the signal in the electrochemical detection (Analytica Chimica Acta, 1207, 339817 (2022))On the other point, understanding the limitation of the signal amplification and uniformity of the nanocarrier, this year, metal-organic framework, a recent uprising nanocarrier, was prepared together with small nanozyme Pt to amplify detection signal for colorimetric biosensor. Here, the nanozyme (NZ) showcased highly sensitive detection limit which is superior to the precedent research of this nanozyme-based immunoassay. (Biosensors and Bioelectronics, 126, 114602, (2022)).Several works have also been done however those works are not yet done in reporting to the society, on how we improve the immunoassay-based biosensor for higher reliability in the detection and how to implement the nanozyme-based detection to the paper-based immunoassay. Those pending writings are the drawback of this year progress.

在该奖学金的最后一年，目标集中在开发用于病毒检测的高灵敏度生物传感器。与前一年相比，开发的显色纳米载体使用封装是不适合的电化学传感系统。然后，我们调整了方法，将适体网络内携带的信号分子螯合在电极上，以放大电化学检测中的信号（Analytica Chimica Acta，1207，339817（2022））另一方面，了解纳米载体的信号放大和均匀性的限制，今年，金属有机框架，最近崛起的纳米载体，与小分子纳米酶Pt一起制备用于比色生物传感器的检测信号放大。在这里，纳米酶（NZ）显示出高灵敏度的检测限，这是上级优于这种基于纳米酶的免疫测定的先前研究。（Biosensors and Bioelectronics，126，114602，（2022））。关于我们如何改进基于免疫测定的生物传感器以获得更高的检测可靠性以及如何将基于纳米酶的检测实施到基于纸的免疫测定，也已经进行了几项工作，但是这些工作尚未向社会报告。这些尚未完成的文件是今年进展的缺点。

项目成果

Impedimetric biosensor of Norovirus with low variance using simple bioconjugation on conductive polymer-Au nanocomposite

使用导电聚合物-Au纳米复合材料上的简单生物共轭构建低方差的诺如病毒阻抗生物传感器

• DOI: 10.1016/j.snb.2022.132390
• 发表时间: 2022
• 期刊: Sensors and Actuators B: Chemical
• 作者: Nasrin Fahmida;Khoris Indra Memdi;Chowdhury Ankan Dutta;Boonyakida Jirayu;Park Enoch Y.
• 通讯作者: Park Enoch Y.

Polymeric Nanoparticle-Laden As Advanced Immunoassay For Virus Detection

载有聚合物纳米颗粒的病毒检测先进免疫测定法

• 发表时间: 2022
• 作者: Indra Memdi Khoris;Akhilesh B. Ganganboina;and Enoch Y. Park
• 通讯作者: and Enoch Y. Park

Development of binary responsive virus biosensor using chromogen-encapsulated polymeric nanocarrier

使用色原包封的聚合物纳米载体开发二元响应病毒生物传感器

• 发表时间: 2021
• 作者: Indra M. Khoris;Akhilesh B. Ganganboina;Enoch Y. Park
• 通讯作者: Enoch Y. Park

Nanocarrier utilization in virus biosensor using chromo-gen-embedded Polymeric Nanoparticles-Laden

使用嵌入发色团的聚合物纳米粒子负载纳米载体在病毒生物传感器中的应用

• 发表时间: 2020
• 作者: Indra M. Khoris;Akhilesh B. Ganganboi and Enoch Y. Park
• 通讯作者: Akhilesh B. Ganganboi and Enoch Y. Park

Self-Assembled Chromogenic Polymeric Nanoparticle-Laden Nanocarrier as a Signal Carrier for Derivative Binary Responsive Virus Detection

• DOI: 10.1021/acsami.1c08813
• 发表时间: 2021-07-30
• 期刊: ACS APPLIED MATERIALS & INTERFACES
• 影响因子: 9.5
• 作者: Khoris, Indra Memdi;Ganganboina, Akhilesh Babu;Park, Enoch Y.
• 通讯作者: Park, Enoch Y.