Signal-amplified two-way detection of multiple viruses using chromogen-embedded nanocarriers

使用显色剂嵌入纳米载体对多种病毒进行信号放大双向检测

基本信息

批准号：
20J22358
负责人：
コリスインドラメンディ
金额：
$ 1.79万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-24 至 2023-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20J22358/
关键词：
Virus detection Signal Amplification Biosensor Aptamer Electrochemical Colorimetric Nanozyme Activity Nanomaterial Virus Detection Signal Enrichment colorimetric Polymer nanomaterial Derivative spectroscopy Analytical Chemistry /

项目摘要

In the last year of this fellowship, the aim focused on developing highly sensitive biosensor for virus detection. Compared to previous year, the developed chromogenic nanocarrier using encapsulation is not suitable for electrochemical sensing system. Then, we adjusted the method and chelating the signal molecules carried within the aptamer network on the electrode to amplify the signal in the electrochemical detection (Analytica Chimica Acta, 1207, 339817 (2022))On the other point, understanding the limitation of the signal amplification and uniformity of the nanocarrier, this year, metal-organic framework, a recent uprising nanocarrier, was prepared together with small nanozyme Pt to amplify detection signal for colorimetric biosensor. Here, the nanozyme (NZ) showcased highly sensitive detection limit which is superior to the precedent research of this nanozyme-based immunoassay. (Biosensors and Bioelectronics, 126, 114602, (2022)).Several works have also been done however those works are not yet done in reporting to the society, on how we improve the immunoassay-based biosensor for higher reliability in the detection and how to implement the nanozyme-based detection to the paper-based immunoassay. Those pending writings are the drawback of this year progress.

在这一奖学金的最后一年中，该目标的重点是开发高度敏感的生物传感器以进行病毒检测。与上一年相比，使用封装开发的发色纳米载体不适用于电化学传感系统。然后，我们调整了电极上适体网络中携带的信号分子的方法，以在电化学检测中放大信号（Analytica Chimica acta，1207，339817（2022）），在其他点上了解了信号放大的限制和均匀的nanocarrior，a neanocarries，yearrier-neyainecriel，yearrier-ynanocarrier-ney narover，纳米载体与小纳米PT一起制备，以扩增比色生物传感器的检测信号。在这里，纳米酶（NZ）展示了高度敏感的检测极限，这比对基于纳米的免疫测定的先例研究优越。（生物传感器和生物电子学，126，114602，（2022））。但是，在向社会报告时尚未完成这些工作，这些作品尚未完成，以提高我们如何改善基于免疫测定的生物传感器，以提高基于免疫测定的生物传感器，以在检测中进行更高的可靠性以及如何实施基于纸张的纸张基于纸张的Immunoosay。这些待处理的著作是今年进步的弊端。

项目成果

期刊论文数量（21）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Impedimetric biosensor of Norovirus with low variance using simple bioconjugation on conductive polymer-Au nanocomposite

使用导电聚合物-Au纳米复合材料上的简单生物共轭构建低方差的诺如病毒阻抗生物传感器

DOI：
10.1016/j.snb.2022.132390
发表时间：
2022
期刊：
Sensors and Actuators B: Chemical
影响因子：
0
作者：
Nasrin Fahmida;Khoris Indra Memdi;Chowdhury Ankan Dutta;Boonyakida Jirayu;Park Enoch Y.
通讯作者：
Park Enoch Y.

Self-Assembled Chromogenic Polymeric Nanoparticle-Laden Nanocarrier as a Signal Carrier for Derivative Binary Responsive Virus Detection