Signal-amplified two-way detection of multiple viruses using chromogen-embedded nanocarriers

在该奖学金的最后一年，目标集中在开发用于病毒检测的高灵敏度生物传感器。与往年相比，采用包封法制备的显色纳米载体并不适合电化学传感系统。然后，我们调整了方法，并在电极上螯合适配体网络内携带的信号分子，以放大电化学检测中的信号（Analytica Chimica Acta, 1207, 339817(2022)）。另一方面，了解纳米载体对信号放大和均匀性的限制，今年，与小纳米酶Pt一起制备了最近兴起的金属-有机框架纳米载体，用于放大比色生物传感器的检测信号。在这里，纳米酶（NZ）显示出高灵敏度的检测限，优于这种基于纳米酶的免疫分析的先例研究。[j] .生物传感器与电子学报，2016,33(2):481 - 481。关于如何改进基于免疫测定的生物传感器以提高检测的可靠性，以及如何将基于纳米酶的检测实现到基于纸的免疫测定中，也已经做了一些工作，但这些工作尚未向社会报告。那些尚未完成的工作是今年进展的不利因素。

In the last year of this fellowship, the aim focused on developing highly sensitive biosensor for virus detection. Compared to previous year, the developed chromogenic nanocarrier using encapsulation is not suitable for electrochemical sensing system. Then, we adjusted the method and chelating the signal molecules carried within the aptamer network on the electrode to amplify the signal in the electrochemical detection (Analytica Chimica Acta, 1207, 339817 (2022))On the other point, understanding the limitation of the signal amplification and uniformity of the nanocarrier, this year, metal-organic framework, a recent uprising nanocarrier, was prepared together with small nanozyme Pt to amplify detection signal for colorimetric biosensor. Here, the nanozyme (NZ) showcased highly sensitive detection limit which is superior to the precedent research of this nanozyme-based immunoassay. (Biosensors and Bioelectronics, 126, 114602, (2022)).Several works have also been done however those works are not yet done in reporting to the society, on how we improve the immunoassay-based biosensor for higher reliability in the detection and how to implement the nanozyme-based detection to the paper-based immunoassay. Those pending writings are the drawback of this year progress.