Signal-amplified two-way detection of multiple viruses using chromogen-embedded nanocarriers

在该奖学金的最后一年，目标集中在开发用于病毒检测的高灵敏度生物传感器。与前一年相比，所开发的包埋式显色纳米载体不适用于电化学传感系统。然后，我们调整了方法，将适体网络中携带的信号分子螯合到电极上，用于电化学检测中的信号放大(Analytica Chimica Acta，1207,339817(2022年))。另一方面，了解到纳米载体信号放大和均匀的局限性，今年我们与小纳米酶铂一起制备了金属-有机骨架纳米载体，用于放大比色生物传感器的检测信号。在这里，纳米酶(NZ)显示出高度灵敏的检测极限，这优于这种基于纳米酶的免疫分析的先例研究。(生物传感器与生物电子学，126,114602，(2022年))。也做了一些工作，但这些工作还没有向社会报告，如何改进基于免疫分析的生物传感器以提高检测的可靠性，以及如何实现基于纳米酶的检测到基于纸张的免疫分析。这些悬而未决的写作是今年进展的缺点。

In the last year of this fellowship, the aim focused on developing highly sensitive biosensor for virus detection. Compared to previous year, the developed chromogenic nanocarrier using encapsulation is not suitable for electrochemical sensing system. Then, we adjusted the method and chelating the signal molecules carried within the aptamer network on the electrode to amplify the signal in the electrochemical detection (Analytica Chimica Acta, 1207, 339817 (2022))On the other point, understanding the limitation of the signal amplification and uniformity of the nanocarrier, this year, metal-organic framework, a recent uprising nanocarrier, was prepared together with small nanozyme Pt to amplify detection signal for colorimetric biosensor. Here, the nanozyme (NZ) showcased highly sensitive detection limit which is superior to the precedent research of this nanozyme-based immunoassay. (Biosensors and Bioelectronics, 126, 114602, (2022)).Several works have also been done however those works are not yet done in reporting to the society, on how we improve the immunoassay-based biosensor for higher reliability in the detection and how to implement the nanozyme-based detection to the paper-based immunoassay. Those pending writings are the drawback of this year progress.