Die Rolle des MATE-Proteins für die Ektomykorrhizasymbiose mit Pilzen der Gattung Tricholoma

MATE蛋白在口蘑属真菌外生菌根共生中的作用

• 批准号: 34466171
• 负责人: Professorin Dr. Erika Kothe
• 金额: --
• 依托单位: Lehrstuhl für Mikrobielle Kommunikation
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/34466171?language=en
• 关键词: Die; Rolle; des; MATE; Proteins

The transporters of the MATE („multidrug and toxic extrusion“) family are part of the stress protection machinery able to excrete a broad variety of compounds from the cell. In the ectomycorrhizal symbiosis, the fungal partner can protect its host tree from environmental stressors by not delivering such toxic agents to the plant. The identification of the MATE encoding gene mte1 from the mycorrhizal basidiomycete Tricholoma vaccinum allowed us to functionally characterize the protein using a yeast system. We could show transport of xenobiotics, plant secondary metabolites and heavy metals. With a newly developed, stable transformation system, the transporter gene can now be over-expressed in Tricholoma, allowing a characterization also under mycorrhizal conditions including transformation of in vitro mutagenized sequences. Since in nature, the fungi will interact with other soil-borne microbes, we would like to identify additional communication molecules for their transport by the Mte1 protein. This work is to be supplemented by investigations performed in another, related fungus, Schizophyllum commune. For this fungus, the genome sequence allowed the identification of two MATE encoding genes, one of which shows evidence for incorporation of selenocysteine, which would modify the activity of the protein. Hence, we want to characterize this new member of the MATE family with special respect to the function of this unusual amino acid.

转运蛋白MATE（“多药和毒性挤压”）家族是应激保护机制的一部分，能够从细胞中排出多种化合物。在外生菌根共生中，真菌伴侣可以通过不向植物输送这些有毒物质来保护其宿主树免受环境胁迫。从菌根担子菌Tricholoma vaccinum中鉴定出MATE编码基因mte1，使我们能够利用酵母系统对该蛋白进行功能表征。我们可以显示外源物、植物次生代谢物和重金属的运输。随着新开发的，稳定的转化系统，转运蛋白基因现在可以在口蘑中过度表达，允许在菌根条件下进行表征，包括体外诱变序列的转化。由于在自然界中，真菌会与其他土壤微生物相互作用，我们希望确定Mte1蛋白运输的其他通讯分子。这项工作将通过对另一种相关真菌裂叶菌（Schizophyllum commune）的调查来补充。对于这种真菌，基因组序列允许鉴定两个MATE编码基因，其中一个显示了硒代半胱氨酸结合的证据，这将改变蛋白质的活性。因此，我们想对MATE家族的这个新成员进行表征，特别考虑到这种不寻常的氨基酸的功能。