Single molecule intracellular intravital imaging of actin dynamics

肌动蛋白动力学的单分子细胞内活体成像

• 批准号: DP160101623
• 负责人: Prof Edna Hardeman
• 金额: $ 32.74万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160101623
• 关键词: Single; molecule; intracellular; intravital; imaging

The project intends to develop imaging technology to visualise fundamental processes in cells within a living animal. The focus will be on the actin cytoskeleton, a dynamic macromolecular machine involved in key cellular processes including cell structure, mobility and division. It is exquisitely sensitive to environmental perturbations, requiring it to be studied in cells in living tissue. The project aims to extend the resolution of live imaging to the single molecule to understand the dynamics of actin assembly with implications for cellular processes that are hijacked in diseases. It also aims to provide a novel assay that may enable testing of the impact of drugs on cellular processes in real time.

该项目旨在开发成像技术，以可视化活动物体内细胞的基本过程。重点将放在肌动蛋白细胞骨架上，这是一种动态的大分子机器，参与关键的细胞过程，包括细胞结构、流动性和分裂。它对环境的干扰非常敏感，需要在活组织的细胞中进行研究。该项目旨在将实时成像的分辨率扩展到单分子，以了解肌动蛋白组装的动力学，以及对疾病中被劫持的细胞过程的影响。它还旨在提供一种新的检测方法，可以实时测试药物对细胞过程的影响。