Molecular Dissection of the Actin Cytoskeleton in Exocytosis Using Intravital Microscopy

使用活体显微镜对胞吐作用中肌动蛋白细胞骨架进行分子解剖

• 批准号: nhmrc : 1079866
• 负责人: Prof Edna Hardeman
• 金额: $ 80.47万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Project Grants
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/molecular-dissection-actin-intravital-microscopy/518706
• 关键词: Molecular; Dissection; Actin; Cytoskeleton; Exocytosis

The proteins actin and tropomyosin form networks in cells that are involved in all of the key processes that are hijacked when a cell becomes cancerous. We discovered that the tropomyosin is the key to the function of a network and have developed drugs that target the cancer-associated tropomyosin. We will examine how the drugs kill cancer cells using a novel method, developed by us, to visualize drug activity in real time, and validate their impact on the target filaments in living tissue.

肌动蛋白和原肌球蛋白在细胞中形成网络，参与细胞癌变时被劫持的所有关键过程。我们发现原肌球蛋白是网络功能的关键，并开发了针对癌症相关原肌球蛋白的药物。我们将使用我们开发的一种新方法来研究药物如何杀死癌细胞，以真实的时间可视化药物活性，并验证它们对活组织中靶丝的影响。