Coordination Funds

协调基金

• 批准号: 386177998
• 负责人: Professor Dr. Markus Rodehutscord
• 金额: --
• 依托单位: Fachgebiet Tierernährung (460a)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/386177998?language=en
• 关键词: Coordination; Funds

The overall objective of this Research Unit is to explore and understand the genetic, epigenetic and non-genetic variations in the formation of inositol phosphate derivatives and myo-inositol by endogenous phytases, as well as their relevance for phosphorus utilisation, microbiome, and animal physiology in the fowl. In the renewal period, we continue testing three overarching hypotheses based on the results of the first period: 1.) phosphorus (P) utilisation by laying hens and myo-inositol metabolism are driven by the bird’s genetic/epigenetic background and the active fraction of the intestinal microbiome, enabling targeted breeding for improved P utilisation; 2.) changes in microbiome, depending on age and feeding, contribute to formation of inositol phosphates and myo-inositol and subsequent physiological and immunological functions; 3.) key physiological features can be identified to scale up the phenotyping of inositol phosphate and myo-inositol metabolism in larger populations. We will conduct three experiments using two high-yielding laying hen strains with a high degree of genetic differentiation. Experiments involve variable dietary supply of free myo-inositol, P and calcium. Projects of the Research Unit obtain their material of investigation from those experiments. Samples of gastrointestinal content, blood, and tissues are analysed using genomic, epigenomic, transcriptomic, proteomic, metabolomic, immunological, microbiological, physiologic, and chemical methods. Besides hypotheses testing in each project, the results will be holistically used in biological network analyses. We aim to identify different biological strategies of P homeostasis and myoinositol metabolism at onset and peak of egg laying in two genetically contrasting laying hen strains. Identification of biomarkers may enable for the consideration of P metabolism in breeding strategies and contribute to a more sustainable management of natural resources and improved animal health.

该研究小组的总体目标是探索和了解内源植酸酶形成肌醇磷酸衍生物和肌醇的遗传、表观遗传和非遗传变异，以及它们与家禽磷利用、微生物组和动物生理学的相关性。在更新期，我们根据第一期的结果继续测试三个总体假设：1.）蛋鸡的磷（P）利用和肌醇代谢是由禽类的遗传/表观遗传背景和肠道微生物组的活性部分驱动的，从而能够进行有针对性的育种以提高磷的利用率； 2.) 微生物组的变化（取决于年龄和喂养）有助于磷酸肌醇和肌醇的形成以及随后的生理和免疫功能； 3.) 可以确定关键的生理特征，以扩大更大人群中磷酸肌醇和肌醇代谢的表型。我们将使用两种具有高度遗传分化的高产蛋鸡品系进行三个实验。实验涉及游离肌醇、磷和钙的可变膳食供应。研究小组的项目从这些实验中获取研究材料。使用基因组、表观基因组、转录组、蛋白质组、代谢组、免疫学、微生物学、生理学和化学方法对胃肠内容物、血液和组织样本进行分析。除了每个项目中的假设检验外，结果将整体用于生物网络分析。我们的目的是确定两种遗传对比蛋鸡品系在产蛋开始和产蛋高峰时 P 稳态和肌醇代谢的不同生物学策略。生物标志物的鉴定可能有助于在育种策略中考虑磷代谢，并有助于更可持续地管理自然资源和改善动物健康。