Study of Host Defense Mechanism against Oral Cancer

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1) The TCR repertoires of TIL in oral SCC were more restricted than PBMC,but still heterogeneous. Careful comparative study revealed that certain V beta-positive T cells were frequently increased in TIL.Although they were polyclonal with different CDR3 sequences, the preferential usage of J beta gene segnents was also observed. Taken together, it is sugestthat the unique T cell subpopulations are amplified possibly as a consequence of antigen-driven selection.2) Using the PCR-based method, cytokine mRNA expression of T cells in oral SCC was examined.Th1type cytokines such as IL-2 and IFN-gamma were more frequently detected that Th2 type cytokines such as IL-4 and IL-5. Furthermore, IFN-gamma were detected more often in patients with marked lymphocyte infiltration. These results suggest that CD4_+ Th1 cells, especially IFN-gamma producing CD4_+ T cells, may participate in active immune responses against oral SCC.3) Extracellular matrix, MMP-1, -2, -3, -9, TIMP-1, -2, and VLA-1-6 are significantly involved in the invasion and metastasizing processes of tumour cells, and that the detection of athese factors is valuable and objective to further evaluate the invasion and metastatic potential of oral SCC,in addition to the conventional histologic grading.