Hybridization and functionalization of biocompatible monomer by in situ polymerization in teeth.

在本项目中，生物相容性粘合剂的体内和体外拉伸结合强度；比较了4-META/MMA-TBB树脂与牙本质的关系，探讨了原位聚合形成的杂化层对牙本质持久粘接的重要性。使用患者无龋牙和因正畸原因计划拔牙。评估拉伸粘结强度，并在粘结测试、抛光脱粘区域或用HCl酸处理表面后立即对所有样品进行切片。对数据进行统计分析，第三磨牙与第一前磨牙、体内标本与体外标本无明显差异。从扫描电镜和透射电镜观察，杂化层的宽度（<正负bbb50 μ m）和粘合剂单体浸渍在10-3溶液中脱矿的重要人牙本质基质中，与提取样品中观察到的宽度基本相似。在口腔内制备的标本中，黏附树脂穿透小管形成树脂标签，在生理压力下不受管内液体的阻碍。在树脂标签上形成的混合层，以防止微渗漏和细菌及其副产品对牙髓的攻击。在牙釉质中也观察到耐酸杂化层。最后，在人牙本质基质的树脂增强混合层底部观察到由聚合的4-META/MMA-TBB树脂包裹的羟基磷灰石晶体。这一观察结果与早期的发现一致，并表明杂交层在维持持久的牙本质结合方面的重要性

In this project, the in vivo and in vitro tensile bond strengths of a biocompatible adhesive ; 4-META/MMA-TBB resin, to human dentin were compared and importance of the hybrid layr, which was created through in situ polymerization of the monomer in teeth, was investigated to obtaining durable bonding. Caries-free teeth present on patients and scheduled for extraction for orthodontic reasons were used. Tensile bond strength was evaluated and all specimens were sectioned immediately after bond testing, after polishing the debonded area or after treating the surfaces with HCl acid. Data were analyzed statistically, and no significant differences could be determined between third molars and first premolars, and in vivo and in vitro specimens. From scanning and transmission electron microscopic observation, widths (<plus-minus>5mum) of hybrid layrs and impregnation of adhesive monomers into 10-3 solution demineralized vital human dentin substrates were essentially similar to those observed in extracted samples. The adhesive resin penetrated tubules to form resin tags, unimpeded by intra-tubular fluid under physiologic pressure in intra-orally prepared specimens. A hybrid layn formed on resin tags to seal against microleakage and pulpal attack by bacteria and their by-products. An acid-resistant hybrid layr was also observed in enamel. Finally, hydroxyapatite crystals that were encapsulated by polymerized 4-META/MMA-TBB resin was observed at the bases of resin-reinforced hybrid layrs in the human dentin substrates. This observation is consistent with earlier findings and indicates the importance of the hybrid layr in maintaining durable dentin bond