Hybridization and functionalization of biocompatible monomer by in situ polymerization in teeth.

本研究比较了生物相容性粘接剂4-Meta/MMA-TBB树脂与牙本质的体内、体外拉伸粘接强度，并探讨了牙本质中单体原位聚合形成的杂化层对获得持久粘接的重要性。使用患者无龋牙齿，并因正畸原因计划拔牙。拉伸粘结强度进行了评价，所有样本进行切片后立即粘结测试，抛光后的脱粘区或用盐酸处理后的表面。数据进行统计学分析，并没有显着差异，可以确定之间的第三磨牙和第一前磨牙，在体内和体外标本。从扫描和透射电子显微镜观察，宽度（<plus-minus>5微米）的混合层和浸渍的粘合剂单体到10-3溶液脱矿的重要人类牙本质基板基本上是相似的，在提取的样品中观察到的。在口腔内制备的标本中，粘合剂树脂渗透小管以形成树脂标签，在生理压力下不受管内流体的阻碍。在树脂标签上形成的混合层，用于密封微渗漏和细菌及其副产物对牙髓的攻击。在釉质中也观察到耐酸的杂化层。最后，在人牙本质基质中的树脂增强混合层的基底处观察到聚合的4-Meta/MMA-TBB树脂包裹的羟基磷灰石晶体。这一观察结果与早期的研究结果一致，并表明混合层在维持持久牙本质粘结方面的重要性

In this project, the in vivo and in vitro tensile bond strengths of a biocompatible adhesive ; 4-META/MMA-TBB resin, to human dentin were compared and importance of the hybrid layr, which was created through in situ polymerization of the monomer in teeth, was investigated to obtaining durable bonding. Caries-free teeth present on patients and scheduled for extraction for orthodontic reasons were used. Tensile bond strength was evaluated and all specimens were sectioned immediately after bond testing, after polishing the debonded area or after treating the surfaces with HCl acid. Data were analyzed statistically, and no significant differences could be determined between third molars and first premolars, and in vivo and in vitro specimens. From scanning and transmission electron microscopic observation, widths (<plus-minus>5mum) of hybrid layrs and impregnation of adhesive monomers into 10-3 solution demineralized vital human dentin substrates were essentially similar to those observed in extracted samples. The adhesive resin penetrated tubules to form resin tags, unimpeded by intra-tubular fluid under physiologic pressure in intra-orally prepared specimens. A hybrid layn formed on resin tags to seal against microleakage and pulpal attack by bacteria and their by-products. An acid-resistant hybrid layr was also observed in enamel. Finally, hydroxyapatite crystals that were encapsulated by polymerized 4-META/MMA-TBB resin was observed at the bases of resin-reinforced hybrid layrs in the human dentin substrates. This observation is consistent with earlier findings and indicates the importance of the hybrid layr in maintaining durable dentin bond