Isolation of monochlorophenol-dechlorinating microorganisms using bio-electrochemical enrichment system with plug flow under anaerobic conditions

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Anaerobic dechlorination is the important reaction in the bioremediation of chlorinated aromatic compounds for decreasing persistency and toxicity of the compounds. There are many reports on the isolation of anaerobic dechlorinator for the polychlorinated aromatic compounds. However, monochlorophenol-dechlorinating anaerobic microorganisms have not been isolated except for few exception. In this study, we have conducted to isolate the monochlorophenol-dechlorinating anaerobic microorganisms. The bio-electrochemical enrichment system, or the enrichment column system with anaerobic plug flow did not enhance the dechlorination of mono-chlorophenol but poly-chlorophenols. Various trials under different conditions suggested that mono-chlorophenol dechlorinating culture did not require other carbon sources such as lactate and formate. Thus, we provided the anaerobic enrichment systems using mono-chlorophenol or phenol as sole source of carbon and energy. The enrichment with phenol resulted in the isolation of anaerobe growing on phenol degradation under sulfate-reducing conditions. The enrichment with 3-chlorophenol was successfully obtained and 3-chlorophenol-dechlorinating anaerobe was identified. These findings expands our knowledge on the mono -chlorophenol dechlorinators and their characteristics.