Isolation of monochlorophenol-dechlorinating microorganisms using bio-electrochemical enrichment system with plug flow under anaerobic conditions

厌氧条件下活塞流生物电化学富集系统分离一氯酚脱氯微生物

• 批准号: 23658272
• 负责人: KATAYAMA Arata
• 金额: $ 2.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658272/
• 关键词: 環境修復; 電気培養集積系; 固体電子メディエータ; モノクロロフェノール; ポリクロロフェノール; 脱塩素; 炭素源; 分解促進; 微生物集積; 電気培養; 還元的脱塩素反応; 微生物群集構造; 回分培養; 脱塩素微生物; 発酵性微生物; クロロフェノール; 電子メディエータ

Anaerobic dechlorination is the important reaction in the bioremediation of chlorinated aromatic compounds for decreasing persistency and toxicity of the compounds. There are many reports on the isolation of anaerobic dechlorinator for the polychlorinated aromatic compounds. However, monochlorophenol-dechlorinating anaerobic microorganisms have not been isolated except for few exception. In this study, we have conducted to isolate the monochlorophenol-dechlorinating anaerobic microorganisms. The bio-electrochemical enrichment system, or the enrichment column system with anaerobic plug flow did not enhance the dechlorination of mono-chlorophenol but poly-chlorophenols. Various trials under different conditions suggested that mono-chlorophenol dechlorinating culture did not require other carbon sources such as lactate and formate. Thus, we provided the anaerobic enrichment systems using mono-chlorophenol or phenol as sole source of carbon and energy. The enrichment with phenol resulted in the isolation of anaerobe growing on phenol degradation under sulfate-reducing conditions. The enrichment with 3-chlorophenol was successfully obtained and 3-chlorophenol-dechlorinating anaerobe was identified. These findings expands our knowledge on the mono -chlorophenol dechlorinators and their characteristics.

厌氧脱氯是氯代芳香族化合物生物修复中的重要反应，可降低其持久性和毒性。厌氧脱氯反应器的分离研究已有很多报道。然而，除了少数例外，一氯酚脱氯厌氧微生物尚未被分离。本研究对一氯酚脱氯厌氧微生物进行了分离。生物电化学富集系统或厌氧推流式富集柱系统对单氯酚的脱氯效果不明显，但对多氯酚的脱氯效果明显。不同条件下的试验表明，一氯酚脱氯培养不需要乳酸、甲酸等其他碳源。因此，我们提供了以一氯酚或苯酚为唯一碳源和能源的厌氧富集系统。富集苯酚导致在硫酸盐还原条件下生长在苯酚降解上的厌氧菌的分离。成功实现了对3-氯苯酚的富集，并对3-氯苯酚脱氯厌氧菌进行了鉴定。这些发现扩展了我们对单氯酚脱氯剂及其特性的认识。

项目成果

Microbial cofinment technology of groundwater contaminated with halogenated organic pollutants

卤代有机污染物污染地下水的微生物共封技术

• 发表时间: 2012
• 作者: Arata Katayama;Zhiling Li;Chunfang Zhang;Dongdong Zhang;Suyin Yang;and Daisuke Suzuki
• 通讯作者: and Daisuke Suzuki

nfluencing factors on monochlorophenol-dechlorinating activities of anaerobic microbial communities enriched from a contaminated river sediment

污染河流沉积物富集厌氧微生物群落一氯酚脱氯活性的影响因素

• 发表时间: 2011
• 作者: Daisuke SUZUKI;Arata KATAYAMA
• 通讯作者: Arata KATAYAMA

微生物の辞典

微生物词典

• 发表时间: 2008
• 作者: 菅江理子;真弓大介;野村暢彦;内山裕夫;中島敏明;中島敏明;渡邉信・西村和子・内山裕夫・奥田徹・加来久敏・広木幹也編(中島敏明 : 共著)
• 通讯作者: 渡邉信・西村和子・内山裕夫・奥田徹・加来久敏・広木幹也編(中島敏明 : 共著)

Influencing factors on monochlorophenol-dechlorinating activities of anaerobic microbial communities enriched from a contaminated river sediment

受污染河流沉积物富集的厌氧微生物群落一氯酚脱氯活性的影响因素

• 发表时间: 2011
• 作者: Daisuke SUZUKI;Arata KATAYAMA
• 通讯作者: Arata KATAYAMA

Long–term Anaerobic Mineralization of PCP in a Continuous–Flow System Using Only Lactate as an External Nutrient

仅使用乳酸作为外部营养物的连续流系统中五氯苯酚的长期厌氧矿化

• 发表时间: 2012
• 作者: 鈴木大典;片山新太;Daisuke SUZUKI;Zhiling LI
• 通讯作者: Zhiling LI