Origin and fate of earliest generated cortical neurons

小鼠大脑皮质中的神经上皮细胞(NE)最初被认为是自我更新的，然后产生基本的祖细胞(BP)。然后，它们中的任何一个通过对称或不对称的分裂产生神经元。这个想法是基于对E12.5左右的小鼠胚胎的观察，以及神经元在早期阶段是如何产生的在很大程度上尚不清楚。在这里，我们在E10.5进行了宫内电操作，并观察了成熟大脑中标记的NE的后代。我们发现亚板细胞、皮质兴奋性神经元以及所有的层以及星形胶质细胞都被标记。这一发现表明，NE在自我更新的同时产生亚板细胞。这也意味着这些类型的细胞有共同的祖细胞起源。

Neuroepithelial cells (NEs) in mouse cortex are thought to initially self renew and then generate basal progenitor cells (BP). Neurons are then generated from either of them by symmetric or asymmetric divisions. This idea is based on observations of mouse embryos at around E12.5 and how neurons are generated at earlier stages remains largely unknown.Here we performed in utero electoroporation at E10.5 and observed progeny of labeled NEs in mature brain. We found that subplate cells, cortical excitatory neurons all all layers as well as astrocyes were labelled. This finding indicates that NEs generate subplate cells concomitantly with their self renewal. It also implies that these types of cells have common progenitor origins.