Study of combined effect of hepatocyte growth factor and mechanical stretching to the peripheral nerve injured muscle

虽然电刺激和拉伸在康复中经常用于治疗失神经肌肉，但尚未建立一种完善的治疗方法。因此，我们使用肌肉去神经动物模型研究拉伸对骨骼肌纤维化和萎缩的影响。坐骨神经去神经早期足底肌肉中与组织纤维化相关的转化生长因子- β 1、α -平滑肌肌动蛋白和胶原蛋白、控制肌肉质量的肌生成抑制素、肌肉特异性泛素激酶环指蛋白-1和肌萎缩素-1表达上调。此外，被动拉伸0.5次/min后的去神经支配肌与未拉伸的去神经支配肌相比，这些因素均显著增加。这些结果可能表明，缓慢的拉伸运动促进失神经肌肉的纤维化和萎缩。

Although the electrical stimulation and stretching are often used in rehabilitation to treat denervated muscles, an accomplished treatment method has not been established. We therefore investigated the effect of stretch to skeletal muscle fibrosis and atrophy using an animal model of muscle denervation. The transforming growth factor-beta 1, alpha-smooth muscle actin and collagen which are related to tissue fibrosis, myostatin, which controls the muscle mass, and muscle RING-Finger Protein-1 and atrogin-1, which are muscle specific ubiquitin kinases, were up-regulated in plantaris muscle in early stage of sciatic nerve denervation. Furthermore, these factors were significant increased after 0.5 times/min passive stretch in denervated muscle compared with non-stretched denervated muscle. These results might suggest that the slow stretching exercise promotes fibrosis and atrophy in denervated muscle.