An epidemiological study of cariogenic and periodontopathic bacteria in children

儿童致龋及牙周病菌流行病学研究

• 批准号: 12672012
• 负责人: TANAKA Shoji
• 金额: $ 2.5万
• 依托单位: Meikai University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672012/
• 关键词: Children; Caries; Periodontal disease; Supragingival plaque; Cariogenic and periodontopathic bacteria; Immunoslot blot assay; Monoclonal antibody; Epidemiological study; 歯肉縁上及び歯肉縁下プラーク; 歯肉縁下及び歯肉縁下プラーク

It has been generally accepted that mutants streptococci is an initiative bacterium in human dental caries. The most common species encountered in human are Streptococcus mutants and Streptococcus sobrinus. Adult periodontal disease is associated with the presence of Porphyromonas gingivalis and Prevotella intermedia. Also, localized juvenile periodontitis is caused by Actinobacillus actinomycetemcomitans. The present study was to determine the presence cariogenic and periodontopathic bacteria in the supragingival plaques of children with different oral health conditions. Tooth number (in df: d-decayed deciduous teeth indicated for filling; f-filled deciduous teeth and DMF: D-Decayed teeth; M-Missing teeth; F-Filled teeth), PMA index (P-papillary gingivitis; M-marginal gingivitis; A-attached gingivitis), OHI (oral hygiene index) and oral malodor of each subject were determined prior to the collection of the supragingival plaques. The detection of the cariogenic bacteria (Streptococcus … More sobrinus) in the supragingival plaques was carried out using ELISA with anti-serum. A significant difference between children with caries and without caries was observed in df tooth number and also in oral malodor in clinical parameters. S. sobrinus in children with caries was significantly higher than that in without caries. In addition, the relationship between the oral malodor in children with caries and the debris index was observed. Periodontopathic bacteria (P. gingivalis, P. intermedia, A. actinomycetemcomitans) in the supragingival plaques were detected using an immunoslot bot assay with monoclonal antibodies specific for each micro-orgasm. The frequencies of the three periodontopathic bacteria in the two subject groups (children with and without caries) tested were not different from each other. P. gingivalis positive subjects, but not P. intermedia, A. actinomycetemcomitans counterparts were correlated to oral malodor. Oral malodor was also correlated to debris index, a component of OHI. The group with a higher OHI showed a higher prevalence of periodontopathic bacteria. For the three periodontopathic bacteria in the subjects tested, P. gingivalis, P. intermedia and A. actinomycetemcomitans positive plaques were not age-related. The supragingival plaques in children can harbor periodontopathic bacteria such as P. gingivalis, P. intermedia and A. actinomycetemcomitans. Less

人们普遍认为，突变链球菌是人类龋齿的一种主动细菌。在人类中最常见的种类是突变链球菌和sobrinus链球菌。成人牙周病与牙龈卟啉单胞菌和中间普雷沃氏菌的存在有关。此外，局部青少年牙周炎是由放线菌引起的。本研究旨在了解不同口腔健康状况儿童龈上斑块中是否存在致龋菌和牙周病菌。在收集龈上斑块之前，测定每个受试者的牙数（df: d-蛀牙；f-补牙；DMF: d-蛀牙；m -缺牙；f-补牙）、PMA指数（p -乳头性牙龈炎；m -边缘性牙龈炎；a -附着性牙龈炎）、OHI（口腔卫生指数）和口腔异味。采用抗血清ELISA法检测龈上斑块中致龋菌（链球菌）。有龋儿童与无龋儿童在牙数及口腔异味等临床指标上均有显著差异。有龋儿童的链球菌数量明显高于无龋儿童。此外，还观察了龋病患儿口腔异味与碎片指数的关系。采用免疫槽法检测龈上斑块中的牙周病细菌（牙龈假单胞菌、中间假单胞菌、放线菌假单胞菌）。三种牙周病细菌的频率在两组（有和没有龋齿的儿童）测试中没有差异。牙龈假单胞菌阳性，而中间假单胞菌、放线菌相对应的假单胞菌与口腔异味相关。口腔异味也与碎片指数（OHI的一个组成部分）相关。OHI高的组牙周病细菌患病率高。对于测试对象中的三种牙周病细菌，牙龈假单胞菌、中间假单胞菌和放线菌假单胞菌阳性斑块与年龄无关。儿童的龈上斑块可窝藏牙周病细菌，如牙龈假单胞菌、中间假单胞菌和放线菌球菌。少

项目成果

Tanaka, S.: "Frequency of reactivity for Porphyromonas gingivalis and Prevotella spp.in supra-and subgingival plaques, and periodontal clinical parameters according to subject age"J Periodontol. 73. 877-885 (2002)

Tanaka, S.：“龈上和龈下菌斑中牙龈卟啉单胞菌和普氏菌的反应频率，以及根据受试者年龄的牙周临床参数”J periodontol。

Nagasaki, M. and Tanaka, S.: "Detection of Porphyromonas gingivalis and Prevotella nigrescens in the subgingival plaques in type 2 diabetes mellitus patients"Jpn J Oral Diag/Oral Med. 14(2). 297-308 (2001)

Nagasaki, M. 和 Tanaka, S.：“2 型糖尿病患者龈下斑块中牙龈卟啉单胞菌和黑普氏菌的检测”Jpn J Oral Diag/Oral Med。

Tanaka, S., et al.: "Distribution of periodontopathic bacteria of the acute inflammation sites in exacerbation and the chronic inflammation sites in adult periodontal patients"Jpn J Oral Diag/Oral Med. 13(2). 304-310 (2000)

Tanaka，S.等人：“成人牙周患者中急性炎症恶化部位和慢性炎症部位的牙周病细菌的分布”Jpn J Oral Diag/Oral Med。

Nagasaki, M., et al.: "An epidemeological survey of serviceman with adult periodontal disease (2) - Relationship between clincal parameter and distribution of periodontopathic bacteria in periodontal patients"National Defence Medical J. 48(10,11). 291-297

长崎M.等：“军人成人牙周病流行病学调查(2)——牙周病患者牙周病菌分布与临床参数的关系”国防医学杂志48(10,11)。

Tanaka, S., et al.: "Frequency of reactivity for Porphyromonas gingivalis and Prevotella spp. in supra- and subgingival plaques, and periodontal clinical parameters according to subject age"J Periodontal. 73(8). 877-885 (2002)

Tanaka, S. 等人：“龈上和龈下菌斑中牙龈卟啉单胞菌和普雷沃氏菌的反应频率，以及根据受试者年龄的牙周临床参数”《牙周杂志》。