Outer membrane vesicles (OMVs)mediated protection of E. coli against effects of human antimicrobial peptides.

革兰氏阴性菌产生外膜囊泡（omv）， omv被认为在毒力、炎症和对包膜应激的反应中起作用。我们研究了人对大肠杆菌的抗菌作用，以评估omv介导的抗菌保护是否发生。人b-防御素（hBD-2）经径向扩散试验显示对大肠杆菌具有抑菌作用，补充纯化的omv可保护大肠杆菌不受hBD-2的抑菌作用。为了研究omv与hBD-2的直接相互作用，本研究使用荧光标记的hBD-2荧光相关光谱（FCS）。FCS检测到omv与hBD-2的直接相互作用。这些发现提供了直接结合细菌omv与hBD-2中和其抗菌活性。

Gram-negative bacteria produce outer membrane vesicles (OMVs), and OMVs have proposed roles in virulence, inflammation, and the response to envelope stress. We studied human antimicrobial effects against E. coli to evaluate whether OMVs-mediated antimicrobial protection occurs. Human antimicrobial peptides, human b-defensin (hBD-2) showed antmicrobial effects against E.coli by radial diffusion assay, and supplimentation with purified OMVs provided protection of E.coli against antimicrobial effects of hBD-2. To examine direct interaction of OMVs to hBD-2, fluorescence correlation spectroscopy (FCS) was used for this study with fluorescence-labeled hBD-2. By FCS, direct interaction OMVs to hBD-2 was detected. These findings offer direct binding bacterial OMVs to hBD-2 neutralized its antimicrobial activity.