Study of effect of atmospheric pressure plasma on biological specimen by using in situ real time electron spin resonance technique

利用原位实时电子自旋共振技术研究常压等离子体对生物样本的影响

• 批准号: 24654191
• 负责人: ISHIKAWA Kenji
• 金额: $ 2.5万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24654191/
• 关键词: プラズマ滅菌; 電子スピン共鳴; フリーラジカル; ミドリカビ胞子; プラズマ殺菌

Atmospheric pressure plasma sterilization of pathogens is now paid much attention for usefulness of no chemicals, electrical operations and so on. The plasma generates many kinds of reactive chemical species such as atomic oxygen, excited oxygen, hydroxyl radical, excited nitrogen, nitrous oxide, etc. However, interactions these species with biological specimens such as fungal spores, microorganisms etc. have not been clarified completely. In particular of intentional exposure of the reactive species to the biological specimen, the author has focused on understanding of free-radicals during plasma sterilization. Employing in situ real time electron spin resonance (ESR) technique, observation of free radical on the fungal spore of Penicillium digitatum has been succeeded. ESR signal decay is linked with inactivation of the spore, therefore the author found the close correlation of the free radical detected by ESR technique with biological response of inactivation on the fungal spores.

大气压等离子体灭菌病原体具有无化学品、电操作等优点，目前已受到广泛关注，等离子体产生原子氧、激发态氧、羟基自由基、激发态氮、一氧化二氮等多种活性化学物种，但这些物种与真菌孢子、微生物等生物样品的相互作用尚未完全阐明。特别是故意暴露的活性物质的生物标本，作者集中在等离子体灭菌过程中的自由基的理解。应用原位真实的时间电子自旋共振（ESR）技术，成功地观察了指状青霉（Penicilliumdigitatum）孢子表面的自由基。ESR信号的衰减与孢子的失活有关，因此作者发现ESR技术检测到的自由基与真菌孢子失活的生物学反应密切相关。

项目成果

Real-time in situ electron spin resonance measurements on fungal spores of Penicillium digitatum during exposure of oxygen plasmas

• DOI: 10.1063/1.4733387
• 发表时间: 2012-06
• 期刊: Applied Physics Letters
• 影响因子: 4
• 作者: K. Ishikawa;H. Moriyama;Hiromasa Tanaka;Kazuhiro Tamiya;H. Hashizume;T. Ohta;Masafumi Ito;Sachiko Iseki;K. Takeda;H. Kondo;M. Sekine;M. Hori

Identification of ESR signals arisen from Penicillium digitatum spores-3

指状青霉孢子 3 产生的 ESR 信号的鉴定

• 发表时间: 2013
• 作者: Hiroko Mizuno;Kenji Ishikawa;Hiromasa Tanaka;Hiroshi Hashizume;Takayuki Ohta;Masafumi Ito;Keigo Takeda;Hiroki Kondo;Makoto Sekine;Masaru Hori
• 通讯作者: Masaru Hori

滅菌表示装置および滅菌装置および青果物表皮の殺菌方法

杀菌显示装置、杀菌装置及果蔬皮的杀菌方法

• 发表时间: 2012

Electron Spin Resonance (ESR) Observation of Radicals on Biological Organism Interacted with Plasmas

电子自旋共振 (ESR) 观察生物有机体与等离子体相互作用的自由基

• DOI: 10.1557/opl.2012.928
• 发表时间: 2012
• 期刊: MRS Online Proceedings Library
• 作者: Kenji Ishikawa;Hiroko Moriyama;Kazuhiro Tamiya;Hiroshi Hashizume;Takayuki Ohta;Masafumi Ito;Sachiko Iseki;Hiromasa Tanaka;Keigo Takeda;Hiroki Kondo;Makoto Sekine and Masaru Hori
• 通讯作者: Makoto Sekine and Masaru Hori

Electron spin resonance study of radical generation during non-thermal plasma blood coagulation

低温血浆凝血过程中自由基产生的电子自旋共振研究

• 发表时间: 2014
• 作者: Kenji Ishikawa;Hiroshi Hashizume;Takayuki Ohta;Masafumi Ito;Hiromasa Tanaka;Keigo Takeda;Satomi Tajima;Hiroki Kondo;Makoto Sekine;and Masaru Hori
• 通讯作者: and Masaru Hori