Systematic analysis on the inter-gene regions of the Escherichia coli genome
Systematic analysis on the inter-gene regions of the Escherichia coli genome
批准号:
24657004
负责人:
KATAYAMA Tsutomu
金额:
$2.66万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31
中文摘要
点击翻译按钮获取中文摘要
英文摘要
In this study, we analyzed the inter-gene regions which have a length of more than 0.5 kb in the Escherichia coli genome. First, based on original analysis using informatics and E. coli genome database, we deduced 94 sites which meet our present study. Next, according to priority determined by further database analysis, we systematically constructed deletion mutants of the regions. One of those mutants was found to have clear sensitivity in cell growth at low temperatures. A specific sequence motif was also found in the region, which was suggested to support cell growth at low temperatures. In parallel, we analyzed the datA region which has a cluster of DnaA-binding sites in an inter-gene region. DnaA is the replication initiator protein and we revealed functional mechanism in datA for regulating DnaA,
期刊论文(50)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
DnaA制御サイクルの再構築による解明:新たなDnaA-ATP加水分解経路と核様体構築因子の役割.
通过重建阐明 DnaA 调节循环:一种新的 DnaA-ATP 水解途径和类核组装因子的作用。
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[片山勉, 加生和寿, 藤光和之, 尾崎省吾, 野口泰徳, 宮崎恵里加, 増田圭美, 末次正幸, 川上広宣.]
通讯作者:
川上広宣.
Reconstitution and molecular anayomy of oriC initiation complexes in E. coli chromosomal replication: ssDNA rescruitmentand roles for IHF and dictinct DnaA oligomers formed on oriC.
大肠杆菌染色体复制中 oriC 起始复合物的重建和分子分析:ssDNA 招募以及 IHF 和 oriC 上形成的特异 DnaA 寡聚物的作用。
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Fujita S, Matsuo T, Ishiura M, and Kikkawa M, Ozaki,S.]
通讯作者:
Ozaki,S.
DnaBヘリカーゼ装着反応におけるDnaAドメインIIIの果たす役割
DnaA 结构域 III 在 DnaB 解旋酶附着反应中的作用
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[Kasho, K., 西村昌洋]
通讯作者:
西村昌洋
九州大学薬学研究院分子生物薬学分野
九州大学药学部分子生物药剂学系
DOI:
--
发表时间:
期刊:
影响因子:
--
作者:
[]
通讯作者:
Finding and molecular analysis of a novel inactivation mechanism for the replication initiator DnaA by a specific chromosomal locus.
发现并分子分析特定染色体位点对复制起始子 DnaA 的新型失活机制。
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[加生和寿, 片山 勉]
通讯作者:
片山 勉
共 39 条
Molecular mechanisms on the replication initiation which leads to loading of the helicase and the regulatory systems for the replication initiation
-
批准号:17H03656
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.15万
-
财政年份:2017
-
负责人:KATAYAMA Tsutomu
-
依托单位:
Molecular analyses on the replication initiation complex and its novel regulatory factors in E. coli
-
批准号:22370064
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$12.06万
-
财政年份:2010
-
负责人:KATAYAMA Tsutomu
-
依托单位:
High-order Structure-based Molecular Mechanisms in Replicational Initiation Complex Formation, DNA Unwinding, and Dissociation
-
批准号:19370077
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.81万
-
财政年份:2007
-
负责人:KATAYAMA Tsutomu
-
依托单位:
Protein Dynamics in Complex Apparatus for the Initiation and Regulation of the Chromosomal Replication
-
批准号:16370081
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$8.64万
-
财政年份:2004
-
负责人:KATAYAMA Tsutomu
-
依托单位:
STRUCTURE AND FUNCTION OF MOLECULRAR SWITCH FOR CHROMOSOMAL REPLICATION
-
批准号:14380330
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$9.79万
-
财政年份:2002
-
负责人:KATAYAMA Tsutomu
-
依托单位:
STUDY ON RATIONAL DEVELOPMENT OF NOVEL ANTIBIOTICS TAGRETING DnaA DOMAIN IV
-
批准号:13558090
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$7.17万
-
财政年份:2001
-
负责人:KATAYAMA Tsutomu
-
依托单位:
REGULATORY MACHINERY FOR THE E. COLI CHROMOSOMAL REPLICATION CYCLE
-
批准号:12680677
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.5万
-
财政年份:2000
-
负责人:KATAYAMA Tsutomu
-
依托单位:
Interaction between the replicational initiator DnaA and the DNA polymerase components
-
批准号:10680616
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.18万
-
财政年份:1998
-
负责人:KATAYAMA Tsutomu
-
依托单位:
Study on molecular structure-function relationship of E..coli DnaA protein
-
批准号:08680695
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$1.54万
-
财政年份:1996
-
负责人:KATAYAMA Tsutomu
-
依托单位:
海外基金