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Development of live cell imaging techniques for cell wall polymers

Development of live cell imaging techniques for cell wall polymers
细胞壁聚合物活细胞成像技术的发展
批准号：
24657034
负责人：
HASHIMOTO Takashi
金额：
$2.66万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
项目状态：
已结题
起止时间：
2012-04-01 至 2014-03-31
来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657034/
关键词：
セルロース可視化蛍光標識植物細胞植物

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英文摘要
Fluorescent probes that track and monitor dynamic status of biopolymers, such as cellulose microfibrils, in living plant cells are essential for next-generation dynamic studies of plant cell wall. In this study, we fused in tandem a signal peptide from cell wall protein (expansin), a crystal cellulose-binding module from cellulose degrading enzyme (cellobiohydrolase from Trichoderma reesei), a membrane-spanning domain of plasma membrane receptor (BRI1), and green fluorescent protein (GFP), and transiently expressed this fusion protein in onion epidermal cells. GFP fluorescence was detected in cortical regions as dotts but not in linear filaments. Detailed observation using transgenic plants is necessary.
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Development of a novel membrane integrity testing method by continuous monitoring using optical fiber sensors.
  • 批准号：
    19K04662
  • 项目类别：
    Grant-in-Aid for Scientific Research (C)
  • 资助金额：
    $2.83万
  • 财政年份：
    2019
  • 负责人：
    HASHIMOTO Takashi
  • 依托单位：
    The University of Tokyo
Investigation of membrane fouling mechanism towards potable water production by membrane filtration against highly turbid surface water in Asian developing countries
  • 批准号：
    26889019
  • 项目类别：
    Grant-in-Aid for Research Activity Start-up
  • 资助金额：
    $1.75万
  • 财政年份：
    2014
  • 负责人：
    HASHIMOTO Takashi
  • 依托单位：
    The University of Tokyo
Moment maps in representation theory and noncommutative invariant theory
  • 批准号：
    26400014
  • 项目类别：
    Grant-in-Aid for Scientific Research (C)
  • 资助金额：
    $2.91万
  • 财政年份：
    2014
  • 负责人：
    HASHIMOTO Takashi
  • 依托单位：
    Tottori University
Action of CRH and pathological relevance in the brain of rat model of PTSD
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