A search for the antisense transcripts that affect higher-order life phenomena in nematodes

寻找影响线虫高阶生命现象的反义转录本

• 批准号: 24657121
• 负责人: NISHIZAWA Mikio
• 金额: $ 2.66万
• 依托单位: Ritsumeikan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24657121/
• 关键词: アンチセンス転写物; RNA; 線虫

An antisense transcript (asRNA) has a sequence that is complementary to its messenger RNA (mRNA). In this study, we tried to clarify the effects of asRNAs on higher-order life phenomena in a nematode. We confirmed that asRNAs were transcribed from a heat shock factor gene and a gene encoding a protein in the Notch signaling pathway. Then, we constructed a library to express transcripts that were complementary to mRNA. Nematodes were fed with the library to express asRNAs inside of the nematodes, and then phenotypic changes in development or fertility were examined. Indeed, developmental retardation or sterility of the nematodes was observed. Therefore, this method may provide a basis to functionally analyze the asRNAs that are involved in development.

反义转录物（asRNA）具有与其信使RNA（mRNA）互补的序列。在这项研究中，我们试图阐明asRNA对线虫中高阶生命现象的影响。我们证实，asRNA转录自热休克因子基因和编码Notch信号通路中的蛋白质的基因。然后，我们构建了一个文库来表达与mRNA互补的转录物。用文库喂养线虫以在线虫内部表达asRNA，然后检查发育或生育力的表型变化。事实上，观察到线虫的发育迟缓或不育。因此，该方法可能为发育相关asRNA的功能分析提供基础。

项目成果

Active hexose correlated compound extends lifespan and increases thermotolerance of nematodes

活性己糖相关化合物可延长线虫的寿命并提高其耐热性

• 发表时间: 2013
• 作者: Okuyama T;Yoshigai E;Ikeya Y;Nishizawa M
• 通讯作者: Nishizawa M

立命館大学 生命科学部生命医科学科医化学研究室ホームページ

立命馆大学生命科学部、生物医学部、医学化学实验室主页

線虫C. elegansの寿命延長と熱耐性に対するAHCCの効果

AHCC 对线虫寿命延长和耐热性的影响

• 发表时间: 2013
• 作者: Okuyama T;Yoshigai E;Ikeya Y;Nishizawa M;奥山哲矢,吉開会美,池谷幸信,西澤幹雄
• 通讯作者: 奥山哲矢,吉開会美,池谷幸信,西澤幹雄

立命館大学 研究者学術情報データベース

立命馆大学研究员学术信息数据库

Active hexose correlated compound extends the lifespan and increases the thermotolerance of nematodes.

活性己糖相关化合物可延长线虫的寿命并提高其耐热性。

• 发表时间: 2013
• 期刊: Functional Foods in Health and Disease
• 影响因子: 1
• 作者: Okuyama T;Yoshigai E;Ikeya Y;Nishizawa M
• 通讯作者: Nishizawa M