Identification of muscle support cells generated during muscledifferentiation

肌细胞生成素诱导肌细胞生成的机制尚不清楚。我们已经成功地从分化中的成肌细胞C2C12中分离出肌细胞生成素阴性的细胞，并通过基因芯片发现这些细胞表达与肌细胞生成素阳性细胞不同的基因组。其中一个基因在肌原纤维附近区域表达，该基因的敲除显著影响了斑马鱼的肌发生和增强了细胞凋亡，支持了该基因和表达该基因的细胞参与肌细胞的维持或存活的观点。这些细胞是否由肌卫星细胞产生也被检测。

Mechanisms of myogenesis induced by myogenin have been elusive. Wehave succeeded to isolate myogenin-negative cells from differentiating myoblast C2C12cells, and have found that those ells express sets of genes different from myogeninpositive cells by microarray. One of these genes is expressed in regions adjacent tomuscle, and knockdown of this gene significantly affected myogenesis and enhancedapoptosis in zebrafish, supporting an idea that this gene and cells expressing this geneare involved in the maintenance or survival of muscle cells. Whether these cells aregenerated from muscle satellite cells was examined.