Study on the production of sex-selected offspring in mammals using fertilization inhibitory effect

利用受精抑制效应研究哺乳动物性别选择后代的产生

• 批准号: 24658234
• 负责人: MINAMI NAOJIRO
• 金额: $ 2.66万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658234/
• 关键词: 産み分け; 遺伝子組換え; マウス; 受精阻害; 細胞間架橋; 性染色体

In the present study, we investigated to produce transgenic mice in which mRNAs were not able to pass through the cytoplasmic bridge during spermatogenesis. It has been reported that 5' and 3' untranslated regions of Smok1 gene prevent mRNA from the passage through the cytoplasmic bridge. However, one male transgenic mouse which carries the 5' and 3' untranslated region of Smock1 produced transgenic offspring, suggesting that these regions do not prevent the passage through the cytoplasmic bridge. In addition, we analyzed the copy number of transgene of another transgenic mouse that were not able to inherit the gene (the gene has fertilization inhibiting effect) to offspring. From the results, it is suggested that fertilization inhibiting effect of the gene depends on the copy number of transgene which is reconstructed in the chromosomes.

在本研究中，我们研究了产生转基因小鼠，其中mRNA在精子发生过程中无法通过细胞质桥。据报道，烟雾基因的5'和3'未翻译区域可防止mRNA通过细胞质桥的通道。但是，一只雄性转基因小鼠携带Smock1的5'和3'未翻译区域产生了转基因后代，这表明这些区域并不能阻止通过细胞质桥的通道。此外，我们分析了另一种无法遗传基因的转基因小鼠的转基因的拷贝数（基因具有抑制效果）。从结果中，提出抑制基因的受精作用取决于染色体中重建的转基因的拷贝数。