Development of a rabies vaccine seed with high productivity and immunogenicity by modification of glycosylation

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In this work, we aimed to identify the rabies virus G protein applicative for an effective rabies vaccine by modification of the N-glycosylation. We found that the street virus G proteins with the multiple N-glycosylation additions did not enhance virus production compared with the G proteins with the single addition. The G protein with five additional N-glycosylation sites was able to produce few progeny viruses, but it recovered the function to produce progeny viruses in the presence of an inhibitor of cellular enzymes associated with N-glycosylation, indicating that this modified G protein may be applicative for development of a safe live vaccine strain with limited replication. Moreover, we found that the N-glycan addition, especially addition at position 194, enhanced the immunogenicity of the street virus G protein.