Construction of attenuated human cytomegalovirus vacccine strain which cannnot replicate in human tissues

不能在人体组织中复制的减毒人巨细胞病毒疫苗株的构建

• 批准号: 24659309
• 负责人: ISOMURA Hiroki
• 金额: $ 2.5万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659309/
• 关键词: サイトメガロウイルス; ヒトサイトメガロウイルス

We constrcuted the attenuated human cytomegalovius vaccine which cannot replicate in human tissues. HCMV DNA replication and late gene transcription are not completely linked. Viral-encoded trans-acting factors are required. Recombinant viruses proficient in major immediate early and early viral gene expression and defective in late gene expression are therapeutic disabled infectious single cycle(DISC)vaccine candidates for the induction of cell-mediated and humoral immunity.

我们成功地制备了不能在人体组织中复制的人巨细胞病毒减毒疫苗。HCMV DNA复制与晚期基因转录不完全相关。需要病毒编码的反式作用因子。重组病毒精通于主要的即刻早期和早期病毒基因表达并且在晚期基因表达中有缺陷，是用于诱导细胞介导的和体液免疫的治疗性失能的感染性单循环（DISC）疫苗候选物。