A challenge of lung regeneration by iPS cells

iPS 细胞对肺再生的挑战

• 批准号: 24659396
• 负责人: SAIJO YASUO
• 金额: $ 2.41万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659396/
• 关键词: iPS 細胞; 肺再生; 肺障害; iPS; 肺スキャフォールド; 肺胞上皮細胞; 分化

We transplanted alveolar differentiated iPS cells into the lung scaffolds. After 12 days culture in vitro, Differentiated iPS cells reconstructed the alveoli and over 10% cells were positive for SPC or Tia. In the bleomycin-induced lung injury model mouse, the cells were transplanted intratracheally. Bronchoalveolar lavage demonstrated IL-6 and TNF-a were drastically suppressed in the differentiated iPS cells group compared to the controls. Fibrotic changes were also suppressed in differentiated iPS cells group. SPC+/PKH26+ or T1a+/PKH26+ cells were observed in the differentiated iPS cells group, but positive cells were quite few. In this study, we demonstrated that alveolar differentiated iPS cells could reconstruct alveoli of the lung scaffold, and ameliorate lung fibrosis in the bleomycin-induced lung injury model mouse.

我们将肺泡分化的iPS细胞移植到肺支架中。体外培养12天后，分化的iPS细胞重建肺泡，10%以上的细胞呈SPC或TIA阳性。在博莱霉素性肺损伤模型小鼠中，将细胞移植到气管内。支气管肺泡灌洗结果显示，与对照组相比，分化的iPS细胞组IL-6和TNF-a受到显著抑制。分化iPS细胞组的纤维化改变也受到抑制。分化后的iPS细胞组可见SPC+/PKH26+或T1A+/PKH26+细胞，但阳性细胞较少。在本研究中，我们证明了肺泡分化的iPS细胞可以重建肺支架的肺泡，并减轻博莱霉素性肺损伤模型小鼠的肺纤维化。

项目成果

Methylation of the KEAP1 gene promoter region in human colorectal cancer.

• DOI: 10.1186/1471-2407-12-66
• 发表时间: 2012-02-13
• 期刊: BMC cancer
• 影响因子: 3.8
• 作者: Hanada N;Takahata T;Zhou Q;Ye X;Sun R;Itoh J;Ishiguro A;Kijima H;Mimura J;Itoh K;Fukuda S;Saijo Y
• 通讯作者: Saijo Y

マウスiPS細胞における肺上皮細胞への分化誘導

诱导小鼠 iPS 细胞分化为肺上皮细胞

• 发表时间: 2013
• 作者: ZHOU QILIANG;YE XULU;SUN RUOWEN,松本吉史、浅野義哉、西條康夫
• 通讯作者: SUN RUOWEN,松本吉史、浅野義哉、西條康夫

A change in the number of CCSP(pos)/SPC(pos) cells in mouse lung during development, growth, and repair.

• DOI: 10.1016/j.resinv.2013.04.006
• 发表时间: 2013-12
• 期刊: Respiratory investigation
• 影响因子: 3.1
• 作者: Ruowen Sun;Qiliang Zhou;Xulu Ye;T. Takahata;A. Ishiguro;H. Kijima;T. Nukiwa;Y. Saijo

Updated overall survival results from a randomized phase III trial comparing gefitinib with carboplatin-paclitaxel for chemo-naive non-small cell lung cancer with sensitive EGFR gene mutations (NEJ002)

• DOI: 10.1093/annonc/mds214
• 发表时间: 2013-01-01
• 期刊: ANNALS OF ONCOLOGY
• 影响因子: 50.5
• 作者: Inoue, A.;Kobayashi, K.;Nukiwa, T.
• 通讯作者: Nukiwa, T.

Differentiation of Mouse Induced Pluripotent Stem Cells Into Alveolar Epithelial Cells In Vitro for Use In Vivo

• DOI: 10.5966/sctm.2013-0142
• 发表时间: 2014-06
• 期刊: STEM CELLS Translational Medicine
• 影响因子: 6
• 作者: Qiliang Zhou;Xulu Ye;Ruowen Sun;Y. Matsumoto;M. Moriyama;Yoshiya Asano;Y. Ajioka;Y. Saijo