Development of a functional diagnostic system for angiosarcoma by surface plasmon resonance

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We applied surface plasmon resonance (SPR) for the analysis of circulating tumor cells (CTC) derived from angiosarcoma. We could recover up to 10 angiosarcoma cells from a mixture of 1 ml of human peripheral blood, mixed with 2,000 angiosarcoma cells and anti-CD146 antibodies. Both angiosarcoma cells and normal vascular endothelial cells expressed VEGF receptors on their surface, and one of two angiosarcoma cell lines phosphorylated their EVGF receptors in response to VEGF. However, only normal endothelial cells showed an SPR signal by VEGF. These results suggest that SPR biosensor may detect CTC of angiosarcoma both sensitively and rapidly.