Anti tumor effect of micro RNA transferred by active targeting liposomes

肺癌和恶性胸膜间皮瘤是难治性疾病。尤其是建立新的有效的治疗方法是改善不能手术转移的肿瘤预后的当务之急。我们发现了对这些疾病具有抑制作用的微RNA(MiRNA)，旨在建立主动靶向脂质体(LIP)选择性地积聚到肿瘤细胞上。在这项研究中，我们计划第一次评估培养的肿瘤细胞和小鼠皮下肿瘤模型中LIP的积累情况，第二次评估miRNA-LIP的抗肿瘤作用。最初，细胞和小鼠模型也没有观察到预期的足够的LIP积累，但我们通过改进LIP、给药条件和模型构建方法，成功地提高了积累效率。然而，我们花了大部分时间在这些过程中，评估LIP进入细胞和开发miRNA-LIP是未来的问题。

Lung cancer and malignant pleural mesothelioma are refractory disease. Particularly, establishment of new effective therapies are urgent business to improve prognosis of inoperable tumor with the metastasis.We have discovered microRNA (miRNA) which works inhibitory to these diseases and aimed at the establishment of active targeting liposome (lip) to accumulate to tumor cells selectively. In this study, we planned to evaluate the accumulation of the lip for the cultured tumor cells and the mouse subcutaneous tumor models for the first and the antitumor effect of the miRNA-lip the second.At first, expected enough accumulation of the lip were not observed with cells and mouse models either, but we were successful for improvement of the accumulation efficiency by improving the lip, administration condition, and model construction methods in detail. However we spent most of the period by these processes, evaluation of the lip taking into cells and developing miRNA-lip are future problems.