Function of newly identified basal lamina structure fractone and regulation of neural stem cell differentiation at subventricular zone

新发现的基底层结构fractone的功能及其对室下区神经干细胞分化的调节

• 批准号: 16390048
• 负责人: NINOMIYA Yoshifumi
• 金额: $ 9.22万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390048/
• 关键词: Basement membrane; Extracellular matrix; Cell differentiation; Type IV collagen; Subventricular zone; Stem cell; Fractone; Laminin; 神経幹細胞

We analyzed molecular components of the new basement membrane-like structure of capillaries in the brain, fractone. We also tried identification and separation of adult neural stem cells existing subventricular zone, and investigated mechanisms of stem cell differentiation.[1]Molecular and cellular analysis of the new basement membrane like structure fractone.We detected the basement mambrane molecules such as laminin and type IV collagen in the mouse fractone by immunohistochemistry. Especially, several laminin chains and type IV collagen chains were present in the fractone, but fibronectin was not expressed there. Moreover, the fractone was distributed around almost all of subventricular zone in the brain and spinal code but it was not present in some areas of the third and forth ventricle. Developmental studies showed that the fractone started its expression around subventricular zone at postnatal day seven, and then expression pattern was limited to lateral part of ventricle at postnatal day 14. Furthermore, we recognized the fractone structure more precisely using immunoelectron-microscopic analysis.[2]Identification and separation of neural stem cells from subventricular zoneWe tried to separate neural stem cells from adult mouse subventricular zones and allowed them to further differentiate into neurons and astrocytes in various conditions. To set-up differentiation conditions, we prepared substrates rich in matrix macromolecules.[3]Control of stem cell differentiation by extracellular matrixWe successfully culture neurospheres prepared from subventricular zone of the lateral ventricles and set-up conditions to further differentiate into neurons and astrocytes.

我们分析了大脑中毛细血管的新基底膜样结构的分子组成，fractone。本实验还尝试了脑室下区成体神经干细胞的鉴定和分离，并探讨了干细胞分化的机制。[1]新的基底膜样结构碎片的分子和细胞分析：采用免疫组织化学方法检测小鼠碎片中的基底膜分子如层粘连蛋白和IV型胶原。特别是，几个层粘连蛋白链和IV型胶原蛋白链存在于fractone，但纤连蛋白不表达。此外，在脑和脊髓的几乎所有室管膜下区都有分布，但在第三和第四脑室的某些区域没有分布。发育研究表明，该蛋白在生后7天开始在脑室下区表达，到生后14天表达范围仅限于脑室外侧部。此外，我们认识到更精确地使用免疫电子显微镜分析的碎片结构。[2]室管膜下区神经干细胞的鉴定与分离我们尝试从成年小鼠室管膜下区分离神经干细胞，并使其在各种条件下进一步分化为神经元和星形胶质细胞。为了建立分化条件，我们制备了富含基质大分子的底物。[3]细胞外基质对干细胞分化的调控我们成功地培养了从侧脑室室管膜下区制备的神经球，并建立了进一步分化为神经元和星形胶质细胞的条件。

项目成果

Loss of expression of type IV collagen alpha5 and alpha6 chains in colorectal cancer associated with the hypermethylat ion of their promoter region

结直肠癌中 IV 型胶原 α5 和 α6 链表达缺失与其启动子区域的高甲基化相关

• 发表时间: 2006
• 期刊: AM J Pathol 168・3
• 作者: Maehara;N.;Chijiiwa;K.;Eto;T.;Funagayama;M.;Uchiyama;S.;Nakashima;S.;Hidaka;H.;Hotokezaka;M.;Ohmuraya M;Takamori H;Motomura Y;Komori H;Masuda Y;Chikamoto A;Okuma T;Maeda K;Maeda K;Hosaka S;Hirota M;Sugita H;Hirota M;Hirota M;Hirota M;Tokunaga E;Takahashi I;Yamamoto M;Ikeda K
• 通讯作者: Ikeda K

Astrocytes express type VIII collagen during the repair process of brain cold injury

• DOI: 10.1016/j.bbrc.2004.03.049
• 发表时间: 2004-04-30
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Hirano, S;Yonezawa, T;Ninomiya, Y
• 通讯作者: Ninomiya, Y

ADAMTS-9 is synergistically induced by interleukin-1beta and tumor necrosis factor alpha in OUMS-27 chondrosarcoma cells and in human chondrocytes.

• 发表时间: 2005
• 期刊: Arthritis and rheumatism
• 作者: K. Demircan;S. Hirohata;K. Nishida;O. Hatipoglu;T. Oohashi;T. Yonezawa;S. Apte;Y. Ninomiya

Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development

• DOI: 10.1242/dev.01037
• 发表时间: 2004-04-01
• 期刊: DEVELOPMENT
• 影响因子: 4.6
• 作者: Pöschl, E;Schlötzer-Schrehardt, U;Mayer, U
• 通讯作者: Mayer, U

Suppression of chndrosarcoma cells by 15-deoxy-Delta 12,14-prostaglandin J2 is associated with altered expression of Bax/Bcl-xL and p21.

15-脱氧-Delta 12,14-前列腺素 J2 对软骨肉瘤细胞的抑制与 Bax/Bcl-xL 和 p21 表达的改变有关。

• 发表时间: 2005
• 期刊: Biochem Biophys Res Commun. 328(2)
• 作者: Shen ZN;Nishida K;Doi H;Oohashi T;Hirohata S;Ozaki T;Yoshida A;Ninomiya Y;Inoue H.
• 通讯作者: Inoue H.