Biological Function of Type VIII Collagen

VIII 型胶原蛋白的生物学功能

基本信息

  • 批准号:
    06044154
  • 负责人:
  • 金额:
    $ 1.6万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for international Scientific Research
  • 财政年份:
    1994
  • 资助国家:
    日本
  • 起止时间:
    1994 至 无数据
  • 项目状态:
    已结题

项目摘要

In order to know which cells or organs produce type VIII collagen, we extracted RNA from various cells and tissues and performed Northern blot analysis using specific cDNAs. Cultured human aortic endothelial cells were found to keep synthesizing alpha1 (VIII) and alpha2 (VIII) chains until tenth passage. These cells synthesize collagen type I,III,and V at the same time. In separate experiment rat aortic smooth muscle cells were tested whether they produce collagens. What we detected was that the cells synthesized 75 kDa bacterial collagenase sensitive materials. Of interest was that production of this materials was enhanced 20 to 30 times by addition of heparin into the culture medium. Currently we are testing whether this bacterial collagenase sensitive material is the alpha-chain of type VIII by Northern blotting analysis. We also demonstrated that mouse keratinocytes produce collagen VIII at the mRNA level using Northern-blotting and in situ hybridization.Several lines of transgenic mice did show strange expression pattern of type VIII collagen gene during development. It seemed that its pattern was strict and developmentally regulated. However, we did not know whether the pattern was artifact or real, since we did not predict the expression pattern ; transient in certain tissues. Direct in situ hybridization was performed using the specific probes for mouse collagen type VIII at the developmental stage and proved that the expression pattern was real.
为了了解哪些细胞或器官产生VIII型胶原,我们从各种细胞和组织中提取RNA,并使用特定cDNA进行北方印迹分析。发现培养的人主动脉内皮细胞持续合成α 1(VIII)和α 2(VIII)链,直到第10代。这些细胞同时合成I型、III型和V型胶原。在单独的实验中,测试大鼠主动脉平滑肌细胞是否产生胶原蛋白。我们检测到的是细胞合成75 kDa的细菌胶原酶敏感物质。感兴趣的是,通过向培养基中添加肝素,该材料的产量提高了20至30倍。目前,我们正在通过北方印迹分析来测试这种细菌胶原酶敏感材料是否是VIII型的α链。Northern印迹和原位杂交结果表明,小鼠角质形成细胞在mRNA水平上表达VIII型胶原,几种转基因小鼠在发育过程中VIII型胶原基因的表达模式存在差异。它的模式似乎是严格的和发展的规定。然而,我们不知道该模式是人为的还是真实的,因为我们没有预测表达模式;在某些组织中是短暂的。用小鼠VIII型胶原特异性探针进行了直接原位杂交,证明了其表达模式是真实的。

项目成果

期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Inoguchi K.,Yoshioka H.,Khaleduzzaman M.,and Ninomiya Y.: "The mRNA for the α(XIX)collagen chain,a new member of FACITs,contain a long unusual 3′ untranslated region and displays many unique splicing variants." J.Biochem.(Tokyo). 117. 137-146 (1995)
Inoguchi K.、Yoshioka H.、Khaleduzzaman M. 和 Ninomiya Y.:“FACIT 的新成员 α(XIX) 胶原链的 mRNA 包含一个长的不寻常的 3 非翻译区,并显示出许多独特的剪接变体。 “ J.Biochem.(东京)。117. 137-146(1995)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Sugimoto M.,Oohashi T.,and Ninomiya Y.: "The two genes,COL4A5 and COL4A6,coding for the two new basement membrane collagen chains,α5(IV) and α6(IV),are located head-to-head in close proximity on chromosome Xq22 and COL4A6 is transcribed from two alternate
Sugimoto M.、Oohashi T. 和 Ninomiya Y.:“编码两个新基底膜胶原链 α5(IV) 和 α6(IV) 的两个基因 COL4A5 和 COL4A6 头对头位于染色体 Xq22 和 COL4A6 上的紧密接近是由两个备用转录的
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Inoguchi K., Yoshioka H., Khaleduzzaman M., and Ninomiya Y.: "The mRNA for the alpha1 (XIX) collagen chain, a new member of FACTTs, contain a long unusual 3'untranslated region and displays many unique splicing variants." J.Biochem. (Tokyo). 117. 137-146
Inoguchi K.、Yoshioka H.、Khaleduzzaman M. 和 Ninomiya Y.:“α1 (XIX) 胶原链的 mRNA 是 FACTT 的新成员,包含一个长的不寻常的 3 非翻译区,并显示出许多独特的剪接变体。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Yoshioka H.,Greenwel P.,Inoguchi K.,Truter S.,Inagaki Y.,Ninimiya Y.,and Ranirez F.: "Structural and functional analysis of the promoter of the human α(XI) collagen gene." J.Biol.Chim.270. 418-424 (1995)
Yoshioka H.、Greenwel P.、Inoguchi K.、Truter S.、Inagaki Y.、Ninimiya Y. 和 Ranirez F.:“人类 α(XI) 胶原蛋白基因启动子的结构和功能分析。”生物化学.270。418-424(1995)
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Oohashi T., Sugimoto M., Mattei M.-G., and Ninomiya Y.: "Identification of a new collagen IV chain, alpha6 (IV), by cDNA isolation and assignment of the gene to chromosome Xq22, which is the same locus for COL4A5." J Biol Chem. 269. 7520-7526 (1994)
Oohashi T.、Sugimoto M.、Mattei M.-G. 和 Ninomiya Y.:“通过 cDNA 分离和将基因分配到染色体 Xq22 来鉴定新的胶原蛋白 IV 链 alpha6 (IV),这与染色体 Xq22 相同
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

NINOMIYA Yoshifumi其他文献

NINOMIYA Yoshifumi的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('NINOMIYA Yoshifumi', 18)}}的其他基金

Anti tumor effect of micro RNA transferred by active targeting liposomes
主动靶向脂质体转移微小RNA的抗肿瘤作用
  • 批准号:
    24659590
  • 财政年份:
    2012
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of Imaging Diagnosis for Lymphedema
淋巴水肿影像诊断的进展
  • 批准号:
    22659323
  • 财政年份:
    2010
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Function of newly identified basal lamina structure fractone and regulation of neural stem cell differentiation at subventricular zone
新发现的基底层结构fractone的功能及其对室下区神经干细胞分化的调节
  • 批准号:
    16390048
  • 财政年份:
    2004
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Function of subendothelial basement membranes in Blood-Brain Barrier
内皮下基底膜在血脑屏障中的功能
  • 批准号:
    14370434
  • 财政年份:
    2002
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functional Analysis of Basement Membrane Collagen Genes
基底膜胶原基因的功能分析
  • 批准号:
    11694280
  • 财政年份:
    1999
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Inhibitory mechanism of vascular endothelial cell proliferation by endostatin
内皮抑素抑制血管内皮细胞增殖的机制
  • 批准号:
    11470274
  • 财政年份:
    1999
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Reconstruction of basement membranes aiming at artificial organs
针对人工器官的基底膜重建
  • 批准号:
    10557113
  • 财政年份:
    1998
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Functional Analysis of Collagen IV by Gene Targeting
通过基因打靶对 IV 型胶原蛋白进行功能分析
  • 批准号:
    09044308
  • 财政年份:
    1997
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Supramolecular Assembly and Function of New Basement Membrane Collagen Molecules
新型基底膜胶原分子的超分子组装及其功能
  • 批准号:
    08457154
  • 财政年份:
    1996
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Biological function of basement membrane
基底膜的生物学功能
  • 批准号:
    07044268
  • 财政年份:
    1995
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for international Scientific Research

相似海外基金

An image-based AI tool to identify stiffness- or age-related mechanotransduction abnormalities in vascular smooth muscle cells
一种基于图像的人工智能工具,用于识别血管平滑肌细胞中与硬度或年龄相关的机械转导异常
  • 批准号:
    BB/Y513994/1
  • 财政年份:
    2024
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Research Grant
Exploration of pathology in Moyamoya disease; co-culture model of vascular endothelial and smooth muscle cells derived from induced pluripotent stem cells
烟雾病病理学探索;
  • 批准号:
    20K22775
  • 财政年份:
    2023
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Grant-in-Aid for Research Activity Start-up
The role of PAR2 and HuR in programming atherosclerotic vascular smooth muscle cells
PAR2和HuR在动脉粥样硬化血管平滑肌细胞编程中的作用
  • 批准号:
    10749319
  • 财政年份:
    2023
  • 资助金额:
    $ 1.6万
  • 项目类别:
Branched-chain Keto-acids and Aerobic Glycolysis in Vascular Smooth Muscle Cells
血管平滑肌细胞中的支链酮酸和有氧糖酵解
  • 批准号:
    10731096
  • 财政年份:
    2023
  • 资助金额:
    $ 1.6万
  • 项目类别:
Novel Calcium Signaling Nanodomains in Vascular Smooth Muscle Cells
血管平滑肌细胞中的新型钙信号纳米结构域
  • 批准号:
    10744522
  • 财政年份:
    2023
  • 资助金额:
    $ 1.6万
  • 项目类别:
Effects of Mechanical Stress and Phenotype Switching on Human Stem Cell-Derived Vascular Smooth Muscle Cells: Modeling Gene Regulatory Networks
机械应力和表型转换对人类干细胞衍生的血管平滑肌细胞的影响:基因调控网络建模
  • 批准号:
    2135907
  • 财政年份:
    2022
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Standard Grant
Epigenetic Regulation of Phenotypic Plasticity of Vascular Smooth Muscle Cells
血管平滑肌细胞表型可塑性的表观遗传调控
  • 批准号:
    RGPIN-2020-04592
  • 财政年份:
    2022
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Discovery Grants Program - Individual
Lesions and loss of smooth muscle cells in brain underlies small vessel disease
大脑中平滑肌细胞的病变和损失是小血管疾病的基础
  • 批准号:
    10527075
  • 财政年份:
    2022
  • 资助金额:
    $ 1.6万
  • 项目类别:
Proteolytic activation of calcium channels: potential target for channel - directed therapeutics in arterial smooth muscle cells
钙通道的蛋白水解激活:动脉平滑肌细胞通道定向治疗的潜在靶点
  • 批准号:
    MR/W001373/1
  • 财政年份:
    2022
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Research Grant
Characterizing the role of MLKL in regulating necroptosis of vascular smooth muscle cells and macrophages
表征 MLKL 在调节血管平滑肌细胞和巨噬细胞坏死性凋亡中的作用
  • 批准号:
    486123
  • 财政年份:
    2022
  • 资助金额:
    $ 1.6万
  • 项目类别:
    Studentship Programs
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了