Tissue Specific Function of Basement Membrane and Heterogeneity in Matrix Molecules

基底膜的组织特异性功能和基质分子的异质性

基本信息

  • 批准号:
    04454564
  • 负责人:
  • 金额:
    $ 4.42万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
  • 财政年份:
    1992
  • 资助国家:
    日本
  • 起止时间:
    1992 至 1993
  • 项目状态:
    已结题

项目摘要

We isolated overlapping cDNAs encoding the human and rabbit alpha4(IV) collagen chains. Charcterization and sequencing of the clones demonstrated the primary structure of the human and rabbit alpha4(IV) collagen chains. The amino acid sequence of the chain is homologous to that of the alpha2(IV) collagen chain. Chromosomal location of the gene was assigned to chromosome 2q35-37.1 by in situ hybridization. Interestingly, the size of the mRNA coding for the alpha4(IV) collagen chain is significantly larger than the other four mRNAs for type IV collagen chains. Comparison of the sequences for these five alpha(IV) chains revealed that there are two subclasses of the gene ; the genes for alpha1, alpha3, and alpha5 chains and genes for alpha2 and alpha4 chains. By these analyzes we predicted that there was one more alpha(IV) chain that belonged to the genes for the alpha2(IV) and alpha4(IV) chains. Using low stringency conditions we successfully isolated a novel cDNA encoding yet another alpha(IV) collagen chain. We designated his new chain as alpha6(IV) chain. Of interest was that the chromosomal location of the gene was assigned to chromosome Xq22, which is the same location as the gene for alpha5(IV) collagen chain. We furtheer isolated several fragments of the gene coding for the alpha6(IV) chain and also the alpha5(IV) chain at the same time. Precise analysis of the fragment of the genes revealed that the genes were located on the same fragement but aligned onto the opposite directions sharing bidirectional promoter in the center of the genes. Further, there was an alternativepromoter approximately 1000 bases downstream of the authentic promoter for the alpha6(IV) gene.
我们分离了编码人和兔α 4(IV)胶原蛋白链的重叠cdna。克隆的鉴定和测序证实了人和兔α 4(IV)胶原蛋白链的初级结构。该链的氨基酸序列与α 2(IV)胶原蛋白链同源。原位杂交将该基因定位在染色体2q35-37.1上。有趣的是,编码alpha4(IV)胶原链的mRNA的大小明显大于其他四种编码IV型胶原链的mRNA。这5条α (IV)链的序列比较表明,该基因有两个亚类;alpha1, alpha3和alpha5链的基因以及alpha2和alpha4链的基因。通过这些分析,我们预测还有一条α (IV)链属于α 2(IV)和α 4(IV)链的基因。在低严格条件下,我们成功地分离了一种新的编码α (IV)胶原蛋白链的cDNA。我们将他的新链命名为alpha6(IV)链。有趣的是,该基因的染色体位置被分配到染色体Xq22上,这与α 5(IV)胶原蛋白链的基因位置相同。我们进一步分离了几个编码alpha6(IV)链和alpha5(IV)链的基因片段。对基因片段的精确分析表明,这两个基因位于相同的片段上,但在相反的方向上排列,在基因中心共享双向启动子。此外,在alpha6(IV)基因的真实启动子下游约1000个碱基处存在一个替代启动子。

项目成果

期刊论文数量(36)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Nagamoto N,Iyama K-I,Kitaoka M,Ninomiya Y: "Rapid expression of collagen type X gene of non-hypertrophic chondrocytes in the grafted chick periosteum demonmstrated by in situ hybridization." J.Histochem Cytochem. 41. 679-684 (1993)
Nagamoto N、Iyama K-I、Kitaoka M、Ninomiya Y:“通过原位杂交证实移植鸡骨膜中非肥大性软骨细胞 X 型胶原蛋白基因的快速表达。”
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Manabu Sugimoto et al: "cDNA isolation and partial gene structure of the human α4(IV)collagen chain" FEBS Letters. 330. 122-128 (1993)
Manabu Sugimoto 等人:“人类 α4(IV) 胶原链的 cDNA 分离和部分基因结构”FEBS Letters 330. 122-128 (1993)。
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    0
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Muragaki Y.,Abe N.,Ninomiya Y.,Olsen B.R.: "The human α1(XV)collagen chain contains a large amino-terminal nontriple-helical domain with a tandem repeat structure." J Biol Chem. 269. 4042-4046 (1994)
Muragaki Y.、Abe N.、Ninomiya Y.、Olsen B.R.:“人类 α1(XV) 胶原链包含具有串联重复结构的大氨基末端非三螺旋结构域。” (1994)
  • DOI:
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  • 影响因子:
    0
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  • 通讯作者:
Sugimoto M, Oohashi T, Yoshioka H, Matsuo N, and Ninomiya Y.: "cDNA isolation and partial gene structure of the human alpha4(IV) collagen chain." FEBS Lett.330. 122-128 (1993)
Sugimoto M、Oohashi T、Yoshioka H、Matsuo N 和 Ninomiya Y.:“人类 α4(IV) 胶原链的 cDNA 分离和部分基因结构。”
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  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Oohashi T.,Sugimoto M.,Mattei M.-G.,Ninomiya Y.: "Identification of a new α6(IV)collagen,by cDNA isolation and assignment of the gene to chromosome Xq22,which is the same locus for COL4A5." J Biol Chem. (in press). (1994)
Oohashi T.、Sugimoto M.、Mattei M.-G.、Ninomiya Y.:“通过 cDNA 分离并将该基因分配到染色体 Xq22(与 COL4A5 的基因座相同)来鉴定新的 α6(IV) 胶原。 “《生物化学杂志》(J Biol Chem)。(出版中)。(1994)
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    0
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NINOMIYA Yoshifumi其他文献

NINOMIYA Yoshifumi的其他文献

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{{ truncateString('NINOMIYA Yoshifumi', 18)}}的其他基金

Anti tumor effect of micro RNA transferred by active targeting liposomes
主动靶向脂质体转移微小RNA的抗肿瘤作用
  • 批准号:
    24659590
  • 财政年份:
    2012
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of Imaging Diagnosis for Lymphedema
淋巴水肿影像诊断的进展
  • 批准号:
    22659323
  • 财政年份:
    2010
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Function of newly identified basal lamina structure fractone and regulation of neural stem cell differentiation at subventricular zone
新发现的基底层结构fractone的功能及其对室下区神经干细胞分化的调节
  • 批准号:
    16390048
  • 财政年份:
    2004
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Function of subendothelial basement membranes in Blood-Brain Barrier
内皮下基底膜在血脑屏障中的功能
  • 批准号:
    14370434
  • 财政年份:
    2002
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Functional Analysis of Basement Membrane Collagen Genes
基底膜胶原基因的功能分析
  • 批准号:
    11694280
  • 财政年份:
    1999
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Inhibitory mechanism of vascular endothelial cell proliferation by endostatin
内皮抑素抑制血管内皮细胞增殖的机制
  • 批准号:
    11470274
  • 财政年份:
    1999
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Reconstruction of basement membranes aiming at artificial organs
针对人工器官的基底膜重建
  • 批准号:
    10557113
  • 财政年份:
    1998
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Functional Analysis of Collagen IV by Gene Targeting
通过基因打靶对 IV 型胶原蛋白进行功能分析
  • 批准号:
    09044308
  • 财政年份:
    1997
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for international Scientific Research
Supramolecular Assembly and Function of New Basement Membrane Collagen Molecules
新型基底膜胶原分子的超分子组装及其功能
  • 批准号:
    08457154
  • 财政年份:
    1996
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Biological function of basement membrane
基底膜的生物学功能
  • 批准号:
    07044268
  • 财政年份:
    1995
  • 资助金额:
    $ 4.42万
  • 项目类别:
    Grant-in-Aid for international Scientific Research

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Characterization of Tagged Type IV Collagen
标记的 IV 型胶原蛋白的表征
  • 批准号:
    10724541
  • 财政年份:
    2023
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Post translational modification of type IV collagen compensates for basement membrane abnormalities in the kidney glomerulus
IV 型胶原的翻译后修饰可补偿肾小球基底膜异常
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arresten(基底膜 IV 型胶原 α1 链的切割片段)在心脏中的作用
  • 批准号:
    19K06428
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整合素靶向 IV 型胶原蛋白衍生肽的多模式抑制
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    9328627
  • 财政年份:
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阐明 Canstatin(IV 型胶原蛋白的裂解片段)在心脏病发展中的作用
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    16K08028
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可视化和阐明力对 IV 型胶原蛋白发育的作用
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    2016
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Differential expression of type IV collagen alpha3 (IV), alpha4 (IV), and alpha5 (IV) chains in non-medullary thyroid carcinoma.
IV 型胶原α3 (IV)、α4 (IV) 和α5 (IV) 链在非髓性甲状腺癌中的差异表达。
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    25462001
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    2013
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使用 IV 型胶原聚集体开发人造血管
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    7568453
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    2008
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Prolyl-3 Hydroxylation of Type IV Collagen in Kidney Development
IV 型胶原蛋白的脯氨酰 3 羟基化在肾脏发育中的作用
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