Analysis of cancer stem cells related to metastasis and recurrence in hepatocellular carcinoma

肿瘤干细胞与肝细胞癌转移和复发的相关性分析

• 批准号: 24659610
• 负责人: OKA MASAAKI
• 金额: $ 2.41万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659610/
• 关键词: 肝癌; 癌幹細胞; 肝細胞癌

The possible existence of plasticity between cancer stem cells and their more differentiated derivative cancer cells was suggested. We successfully induced cancer stem-like sphere cells from human HCC cell lines with specified medium. Poorly differentiated HCC derived SK-HEP-1 and undifferentiated HCC derived HLE cell lines efficiently formed spheres (SK-sphere and HLE-sphere). In the presence of several anti-cancer drugs except sorafenib, the cell viability of SK-sphere and HLE-sphere were significantly higher than that of parental cells. SK-sphere showed higher HIF1A mRNA expression, more CD44 variant-positive cells, and lower ROS production compared to parental cells. Integrated analysis showed that HIF1A and its downstream genes were up-regulated in SK-sphere. Finally, e identified several new marker and therapy target candidates (mRNA, microRNA, and protein) to cancer stem cells by integrated omics analysis.

提示肿瘤干细胞与其分化程度更高的衍生癌细胞之间可能存在可塑性。我们成功地从人肝癌细胞系中诱导出肿瘤干细胞样球细胞。低分化HCC衍生的SK-HEP-1和未分化HCC衍生的HLE细胞系有效地形成球体（SK球体和HLE球体）。在除索拉非尼外的几种抗癌药物存在下，SK-球体和HLE-球体的细胞活力显著高于亲本细胞。SK-球显示更高的HIF 1A mRNA表达，更多的CD 44变体阳性细胞，和较低的ROS产生相比，亲本细胞。综合分析表明，HIF 1A及其下游基因在SK-球中表达上调。最后，我们通过整合组学分析鉴定了几种新的肿瘤干细胞标记物和治疗靶点候选物（mRNA、microRNA和蛋白质）。

项目成果

ヒト肝細胞癌細胞株から誘導した癌幹細胞様細胞の抗癌剤耐性

人肝癌细胞系来源的癌症干细胞样细胞的抗癌耐药性

• 发表时间: 2013
• 作者: 恒富 亮一;吉村 清;橋本 憲輝;渡邊 裕策;岡 正朗
• 通讯作者: 岡 正朗

ヒト膵癌細胞株を用いた癌幹細胞が豊富な細胞集団の誘導

使用人胰腺癌细胞系诱导富含癌症干细胞的细胞群

• 发表时间: 2013
• 作者: 吉村 清;渡邊 裕策;恒富 亮一;新藤 芳太郎;前田 祥成;松井 洋人;前田 訓子;橋本 憲輝;鈴木 伸明;上野 富雄;山本 滋;吉野 茂文;硲 彰一;岡 正朗
• 通讯作者: 岡 正朗

血清を用いたCpG island methylator phenotype (CIMP)による予後評価への応用

血清 CpG 岛甲基化表型 (CIMP) 在预后评估中的应用

• 发表时间: 2012
• 作者: 吉村清;Ryouichi Tsunedomi;Kiyoshi Yoshimura;斎藤 裕;吉村清;斎藤 裕;飯田通久
• 通讯作者: 飯田通久

Susceptibility of hepatoma-derived cells to histone deacetylase inhibitors is associated with ID2 expression.

• DOI: 10.3892/ijo.2013.1811
• 发表时间: 2013-04
• 期刊: International journal of oncology
• 影响因子: 5.2
• 作者: Tsunedomi R;Iizuka N;Harada S;Oka M
• 通讯作者: Oka M

Overexpression of MCM6 is associated with poor prognosis of hepatocellular carcinoma

MCM6过表达与肝细胞癌不良预后相关

• 发表时间: 2013
• 作者: Michihisa Iida;Norio Iizuak;Ryouichi Tsunedomi;Kazuhiko Sakamoto;Takao Tamesa;Shoichi Hazama;Masaaki Oka
• 通讯作者: Masaaki Oka