Novel regulator of gene expression in DRG in a neuropathic pain

神经性疼痛 DRG 基因表达的新型调节因子

• 批准号: 24659688
• 负责人: NOGUCHI Koichi
• 金额: $ 2.41万
• 依托单位: Hyogo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659688/
• 关键词: 脊髄神経節; 遺伝子発現; Artemin; TRPV1; TRPA1; 神経障害性疼痛; 炎症性疼痛; 後根神経節; DRG; 痛覚過敏

We found the following findings. 1. Artemin increases locally in skin over long periods of time, whereas NGF shows transient increases after peripheral inflammation. 2. Artemin increases in peripheral sciatic nerve following to the Wallerian degeneration after nerve injury. 3. Repeated artemin injections changed the gene expressions of TRPV1 and TRPA1 in DRG neurons. 4. The co-localization between TRPV1/A1 and GFRalpha3 was higher than that between TRPV1/A1 and TrkA. 5. Continuous artemin injection into the plantar surface caused mechanical and heat hyperalgesia. 6. Artemin increases TRPV1/A1 expression and Ca activity in primary cultured DRG neurons. 7. TRPV1/A1 expression was regulated by artemin through p38 phosphorylation in primary afferent.We conclude that peripheral tissue-derived artemin synthesis is important regulator of TRPV1/A1 expression in DRG neurons following peripheral inflammation and nerve injury.

我们发现了以下发现。1.青蒿素在皮肤局部长期升高，而NGF在外周炎症后表现为一过性升高。2.周围坐骨神经损伤后瓦勒氏变性后，青蒿素含量增加。3.反复注射青蒿素改变了DRG神经元TRPV1和TRPA1的基因表达。4.TRPV1/A1与GFRalpha3之间的同源性高于TRPV1/A1与TrkA之间的同源性。5.足底持续注射青蒿素可引起机械性和热敏性痛觉过敏。6.青蒿素可增加原代培养的背根节神经元TRPV1/A1的表达和钙的活性。结论：外周组织源性青蒿素合成是外周炎症和神经损伤后DRG神经元TRPV1/A1表达的重要调节因子。

项目成果

Etodolac activates and desensitizes transient receptor potential ankyrin 1

依托度酸激活瞬时受体电位锚蛋白 1 并使之脱敏

• DOI: 10.1002/jnr.23274
• 发表时间: 2013
• 期刊: J Neurosci Res
• 影响因子: 4.2
• 作者: Wang S;Dai Y;Kogure Y;Yamamoto S;Zhang W;Noguchi K
• 通讯作者: Noguchi K

Leukotriene synthases and the receptors in nociceptive pathway contribute to the neuropathic and inflammatory pain

白三烯合酶和伤害性通路中的受体导致神经性疼痛和炎性疼痛

• 发表时间: 2012
• 作者: Okubo M;Yamanaka H;Kobayashi K;Kanda H;Dai Y;Noguchi K.;野口光一;野口光一;野口光一;野口光一;野口光一;Noguchi K
• 通讯作者: Noguchi K

痛みのScience and Practice

疼痛的科学与实践

• 发表时间: 2013
• 作者: Okubo M;Yamanaka H;Kobayashi K;Kanda H;Dai Y;Noguchi K.;野口光一;野口光一;野口光一;野口光一;野口光一;Noguchi K;宮川慈子,野口光一
• 通讯作者: 宮川慈子,野口光一

Up-regulation of platelet-activating factor synthases and its receptor in spinal cord contribute to development of neuropathic pain following peripheral nerve injury.

• DOI: 10.1186/1744-8069-8-8
• 发表时间: 2012-02-02
• 期刊: Molecular pain
• 影响因子: 3.3
• 作者: Okubo M;Yamanaka H;Kobayashi K;Kanda H;Dai Y;Noguchi K
• 通讯作者: Noguchi K

Leukotriene enhances NMDA induced inward currents in dorsal horn neurons of rat spinal cord after peripheral nerve injury.

白三烯增强周围神经损伤后大鼠脊髓背角神经元中 NMDA 诱导的内向电流。

• 发表时间: 2013
• 作者: Kiyoyuki Y;Yamanaka H;Kobayashi K;Okubo M;Noguchi K;Taniguchi W;Nishio N;Nakatsuka T.
• 通讯作者: Nakatsuka T.