Utility value of blastocyst biopsy for preimplantation genetic diagnosis

用于植入前遗传学诊断(PGD)的胚胎活检通常在卵裂期进行，但这往往对胚胎存活率有害。胚泡的滋养胚层活检似乎侵袭性较小，因为内细胞团在活检后仍然完好无损。在本研究中，可以很容易地活检出几个滋养外胚层细胞，所有的胚泡在活检后都会再次扩张。经Colcemid处理的部分囊胚细胞有丝分裂明显中断，Hoechst和碘化丙啶染色证实了这一药效。另一方面，与淋巴细胞FISH一样，整个染色体涂染探针的荧光信号也很强。滋养层活组织检查和有丝分裂期细胞检查对PGD可能是有用的。

Embryo biopsy intended for preimplantation genetic diagnosis (PGD) is generally performed on cleavage stage, but it tend to be harmful for embryo viability. Trophectoderm biopsy from blastocyst is seem to be less invasive, since inner cell mass is remain intact after biopsy. In this study it could be easily biopsied several number of trophectoderm cells and all of blastocysts expanded again after biopsy. Some blastocyst cells treated by Colcemid were significantly interrupted mitosis, which drug efficacy was confirmed by Hoechst and Propidium iodide staining. On the other hand, fluorescent signals of whole chromosome painting probes were strongly detectable as for lymphocyte FISH. Trophectoderm biopsiy followed by examination using mitotic stage cells was likely to useful for PGD.