Differentiated adipose-derived stem cells promote reinnervation of rat skin flaps

本研究的目的是阐明从脂肪源性干细胞（ASCs）分化的雪旺细胞（SC）样细胞是否能促进皮肤皮瓣的皮神经再生。32只大鼠双侧岛状腹股沟皮瓣升高。将ASCs、从ASCs分化的sc样细胞（dASCs）或SCs注射到皮瓣中，免疫组织化学方法评估再神经密度。虽然没有发现移植细胞的长期存活，但dASCs和SCs显著增加了皮瓣周围的再神经密度，这在无神经支配的皮瓣中更为明显。另一方面，ASC对周围神经再生的影响无统计学意义。在皮瓣中心，无论皮瓣是否有神经支配，各组再神经支配密度均无统计学意义的增加。除了神经支配皮瓣的应用外，dASC移植治疗可能是改善皮瓣感觉恢复的新途径。

The aim of this study was to elucidate if Schwann cell (SC)-like cells differentiated from adipose-derived stem cells (ASCs) could improve the cutaneous nerve regeneration in skin flaps. Island groin flaps were elevated bilaterally in 32 rats. ASCs, SC-like cells differentiated from ASCs (dASCs), or SCs were injected to the flaps, and the reinnervation density was immunohistochemically assessed.Although a long-term survival of the transplanted cells was not found, dASCs and SCs significantly increased reinnervation density in the periphery of flaps, and this was more pronounced in noninnervated flaps. On the other hand, ASC showed no statistically significant effect on the peripheral reinnervation. In the center of flap, there was no statistically significant increase in reinnervation density in all groups irrespective of flap innervation.In addition to the use of innervated flaps, dASC transplantation therapy could be a new approach to improve the sensory recovery of skin flaps.