Bacteriostatic effects of neutrophil gelatinase-associated lipocalin against clinically identified bacteria

中性粒细胞明胶酶相关脂质运载蛋白对临床鉴定细菌的抑菌作用

• 批准号: 24659801
• 负责人: MORISAKI HIROSHI
• 金额: $ 2.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659801/
• 关键词: 感染症; NGAL; 静菌効果; lipocalin-2; bacteria; infection

We previously focused on bacteriostatic effects of neutrophil gelatinase-associated lipocalin (NGAL), known as a biomarker of acute kidney injury, and investigated the interaction between this protein and intestinal microflora. The present study demonstrated that bacteriostatic effects of NGAL against bacteria, which is frequently found in clinical situations. As a result, gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae and Enterobacter cloacae was investigated to examine the bacteriostatic effects of NGAL in vitro. To detect bacterial growth, real-time PCR by using bacteria specific primer was performed. Although 1 mcg NGAL did not prevent the growth of these bacteria in vitro, 15 mcg NGAL tended to inhibit the growth of Escherichia coli, but it did not reach statistically significance. In conclusion, the present study demonstrated that high concentration NGAL might be able to inhibit the growth of Escherichia coli.

我们以前专注于中性粒细胞明胶酶相关脂质运载蛋白（NGAL）的抑菌作用，被称为急性肾损伤的生物标志物，并研究了这种蛋白质和肠道微生物菌群之间的相互作用。 目前的研究表明，NGAL对临床常见的细菌具有抑菌作用。 结果，革兰氏阴性菌如大肠杆菌、肺炎克雷伯菌和阴沟肠杆菌进行了研究，以检查NGAL的体外抑菌作用。 为了检测细菌生长，使用细菌特异性引物进行实时PCR。 虽然1 mcg NGAL在体外不能阻止这些细菌的生长，但15 mcg NGAL倾向于抑制大肠杆菌的生长，但没有达到统计学显著性。 实验结果表明，高浓度的NGAL对大肠杆菌的生长有一定的抑制作用。