Establishment of a novel screening system to isolate PTH-like osteogenic small compounds

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Parathyroid hormone (PTH) has been shown to be a effective therapeutic agents for bone diseases such as osteoporosis. However, PTH has two major problems; high cost and limitation of term due to its possible side effect. Thus, we have attempted to contribute to development of screening systems that can allow us to explore the small compounds to mimic osteogenic action of PTH. We found that an osteoblastic cell line, SaOS2, is useful and suitable for the screening system. We also attempted to identify the target genes of PTH. Our data suggest that Wnt5a would be a target gene of PTH during bone formation. Our study suggest that a screening system in which we can determine the Wnt5a gene promoter activity would be helpful for developing the therapeutic bone forming small compounds.