Understanding of molecular basis by which Indian hedgehog signaling regulates osteoblast differentiation

了解印度刺猬信号调节成骨细胞分化的分子基础

• 批准号: 19390473
• 负责人: NISHIMURA Riko
• 金额: $ 12.06万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19390473/
• 关键词: 骨芽細胞; 未分化間葉系細胞; 骨形成; インディアンヘッジホッグ; 細胞内シグナリング; 転写因子; PTHrP; Ihh; Gli; Runx2

Indian hedgehog (Ihh) has been shown to play important roles in development of several tissues including skeletal tissues. In particular, Ihh conducts bone formation by regulating osteoblast and chondrocyte differentiation. In this study, to understand the mechanisms of Ihh, we investigated mechanisms and roles of Ihh signaling pathway. We found that Ihh controls expression and function of transcription factors, Gli family members, thereby regulating osteoblast differentiation. Furthermore, we demonstrated that Ihh signaling collaborates with Bone morphogenetic protein 2 (BMP2) signaling and Runx2, and that this cross-talk enhances osteoblast differentiation.

印度刺猬(IHH)已被证明在包括骨骼组织在内的几种组织的发育中发挥着重要作用。特别是，IHH通过调节成骨细胞和软骨细胞分化来进行骨形成。为了了解IHH的发病机制，本研究对IHH信号通路的机制和作用进行了研究。我们发现IHH调控转录因子Gli家族成员的表达和功能，从而调节成骨细胞的分化。此外，我们还证明了IHH信号与骨形态发生蛋白2(BMP2)信号和Runx2信号协同作用，并且这种相互作用促进了成骨细胞的分化。

项目成果

分子生物硬組織研究のイノベーション

分子生物学硬组织研究的创新

• 发表时间: 2008
• 作者: 西村理行;ら
• 通讯作者: ら

Ihh/Gli2 signaling promotes osteoblast differentiation by regulating Runx2 expression and function

Ihh/Gli2 信号通过调节 Runx2 表达和功能促进成骨细胞分化

• 发表时间: 2007
• 期刊: Mol Biol Cell 18
• 作者: Shimoyama A;Wada M;Ikeda F;Hata K;Matsubara T;Nifuji A;Noda M;Amano K;Yamaguchi A;Nishimura R;Yoneda T
• 通讯作者: Yoneda T

Osterix Functions as Downstream of Runx2 and Msx2 During BMP2-Regulated Osteoblastogenesis.

Osterix 在 BMP2 调节的成骨细胞生成过程中充当 Runx2 和 Msx2 的下游。

• 发表时间: 2007
• 作者: Matsubara T;Kumiko K;Hata K;Yamaguchi A;Aburatani H;Nishimura R;Yoneda T
• 通讯作者: Yoneda T

Sox9 family members negatively regulate maturation and calcification of chondrocytes through up-regulation of PTHrP.

Sox9 家族成员通过上调 PTHrP 负向调节软骨细胞的成熟和钙化。

• 发表时间: 2009
• 期刊: Mol Biol Cell 20
• 作者: Amano K;Hata K;Suigita A;Takigawa Y;Ono K;Wakabayashi M;Kogo M;Nishimura R;Yoneda T
• 通讯作者: Yoneda T

Msx2 Promotes Late Stages of Chondrocyte Differentiation by Up-regulating Ihh Expression.

Msx2 通过上调 Ihh 表达促进软骨细胞分化的晚期阶段。

• 发表时间: 2007
• 作者: Amano K;Ichida F;Sugita A;Hata K;Kogo M;Nishimura R;Yoneda T
• 通讯作者: Yoneda T