Challenge for SHED-based Hematopoietic Regeneration

基于 SHED 的造血再生面临的挑战

• 批准号: 24659815
• 负责人: YAMAZA Takayoshi
• 金额: $ 2.41万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659815/
• 关键词: 乳歯幹細胞; 造血機能; 再生医療; エリスロポイエチン; 骨髄ニッチ; エリスロポエチン

Erythropoietin (EPO) Receptor was expressed on stem cells from human exfoliated deciduous teeth (SHED). EPO-stimutated SHED unregulated Stat5 phospholiration. EPO-stimulated SHED were capable of increasing the formation of hematopietic niches compared to non-stimulated SHED when SHED were subcutanously transplanted with hydroxyapatite carriers into immunocompromised mice. In the increase hematopoietic niche, hematopoietic cells were found by immunofluorescent and flow cytometric analyses. Taken together, these data suggested that EPO is able to induce the capacity of hematopoiesis through SHED.

促红细胞生成素（EPO）受体在人脱落乳牙干细胞（SHED）上表达。EPO刺激的SHED不受调节的Stat5磷脂酶。当将SHED与羟基磷灰石载体皮下移植到免疫功能低下的小鼠中时，EPO刺激的SHED与未刺激的SHED相比能够增加造血壁龛的形成。免疫荧光和流式细胞仪检测发现造血细胞在造血微环境中增多。综上所述，这些数据表明EPO能够通过SHED诱导造血能力。

项目成果

Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine.

• DOI: 10.1371/journal.pone.0051777
• 发表时间: 2012
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Ma L;Makino Y;Yamaza H;Akiyama K;Hoshino Y;Song G;Kukita T;Nonaka K;Shi S;Yamaza T
• 通讯作者: Yamaza T

Trans-differentiation capacity into hepatocyte-like cells of stem cells from human exfoliated deciduous teeth

人脱落乳牙干细胞向肝细胞样细胞的转分化能力

• 发表时间: 2012
• 作者: Alatas FS;Yamaza T;Yamaza H;Hayashida M;Yanagi Y;Kukita T;Nonaka K;Ohga S;Taguchi T
• 通讯作者: Taguchi T

矯正的歯の移動におけるアレルギー誘導性歯根吸収促進機構

过敏引起的正畸牙移动牙根吸收促进机制

• 作者: 村田直久;五百井秀樹;大内雅博;合島怜央奈;沖雄二;山座孝義;高橋一郎;城戸瑞穂
• 通讯作者: 城戸瑞穂

凍結ヒト歯髄組織の臨床応用の可能性

冷冻人牙髓组织的潜在临床应用

• 作者: 馬蘭;山座孝義;星野慶弘;山座治義;野中和明;久木田敏夫;山座孝義
• 通讯作者: 山座孝義

Trans- differentiation and spheroid formation of hepatocyte-like cells of stem cells from human exfoliated deciduous teeth

人脱落乳牙干细胞肝细胞样细胞的转分化和球体形成

• 发表时间: 2012
• 作者: Alatas FS;Takayoshi Yamaza;Haruyoshi Ya maza;Toshio Kukita;Kazuaki Nonaka;Shouici Ohga;Tomoaki Taguchi
• 通讯作者: Tomoaki Taguchi