Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine.
Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine.
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DOI：
10.1371/journal.pone.0051777
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Yamaza T
中科院分区：
文献类型：
--
作者：
Ma L;Makino Y;Yamaza H;Akiyama K;Hoshino Y;Song G;Kukita T;Nonaka K;Shi S;Yamaza T
人类脱落乳牙被认为是再生治疗的一个有前途的来源，因为它们含有来自人类脱落乳牙（SHED）的独特的出生后干细胞，具有自我更新能力、多能性和免疫调节功能。然而，乳牙保存技术尚未发展。本研究的目的是评估人类脱落乳牙牙髓组织的冷冻保存是一个可回收的和实用的SHED来源的细胞为基础的治疗。从冷冻保存超过2年（25-30个月）的乳牙牙髓组织中分离的SHED（SHED-Cryo）具有与从新鲜组织中分离的SHED（SHED-Fresh）相似的干细胞特性，包括克隆形成、自我更新、干细胞标记物表达、多能性、体内组织再生能力和体外免疫调节功能。为研究SHED-Cryo对免疫性疾病的治疗效果，将SHED-Cryo静脉移植到系统性红斑狼疮（SLE）模型MRL/lpr小鼠中。系统性SHED-Cryo移植改善SLE样疾病，包括寿命短，自身抗体水平升高和肾炎样肾功能不全。SHED-Cryo修正了MRL/lpr小鼠中全身和局部增加的分泌白细胞介素17的辅助性T细胞。SHED-Cryo-移植也能够恢复MRL/lpr小鼠长骨中的骨质疏松骨减少。此外，SHED-Cryo介导的组织工程诱导免疫功能低下小鼠关键颅骨骨缺损部位的骨再生。SHED-Cryo移植对免疫和骨骼疾病的治疗效果与SHED-Fresh相似。这些数据表明，乳牙牙髓组织的冷冻保存为基于干细胞的免疫治疗和再生医学中的组织工程提供了合适的和理想的方法。
Human exfoliated deciduous teeth have been considered to be a promising source for regenerative therapy because they contain unique postnatal stem cells from human exfoliated deciduous teeth (SHED) with self-renewal capacity, multipotency and immunomodulatory function. However preservation technique of deciduous teeth has not been developed. This study aimed to evaluate that cryopreserved dental pulp tissues of human exfoliated deciduous teeth is a retrievable and practical SHED source for cell-based therapy. SHED isolated from the cryopreserved deciduous pulp tissues for over 2 years (25–30 months) (SHED-Cryo) owned similar stem cell properties including clonogenicity, self-renew, stem cell marker expression, multipotency, in vivo tissue regenerative capacity and in vitro immunomodulatory function to SHED isolated from the fresh tissues (SHED-Fresh). To examine the therapeutic efficacy of SHED-Cryo on immune diseases, SHED-Cryo were intravenously transplanted into systemic lupus erythematosus (SLE) model MRL/lpr mice. Systemic SHED-Cryo-transplantation improved SLE-like disorders including short lifespan, elevated autoantibody levels and nephritis-like renal dysfunction. SHED-Cryo amended increased interleukin 17-secreting helper T cells in MRL/lpr mice systemically and locally. SHED-Cryo-transplantation was also able to recover osteoporosis bone reduction in long bones of MRL/lpr mice. Furthermore, SHED-Cryo-mediated tissue engineering induced bone regeneration in critical calvarial bone-defect sites of immunocompromised mice. The therapeutic efficacy of SHED-Cryo transplantation on immune and skeletal disorders was similar to that of SHED-Fresh. These data suggest that cryopreservation of dental pulp tissues of deciduous teeth provide a suitable and desirable approach for stem cell-based immune therapy and tissue engineering in regenerative medicine.
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