Genomic analysis of the 11q13.3 amplicon navigation microdissection in oral cancer

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The aim is to develop the methods that detect the genomic structure abnormality in oral cancer cell with high-level genomic abnormality. We were confirming the satisfactory data of CGH microarray analysis using extracted 100ng input DNA from a formalin fixation paraffin embedded (FFPE) tissue. Moreover, we were confirming the satisfactory data of CGH microarray analysis using extracted DNA from a sample mounted in slide-glass which coverslip on the slide were removed by soaking the slide in xylene after immunohistostaining. To detect the genomic abnormality using smaller quantity of input DNA, we performed the targeted next-generation sequencing. We detected the genomic abnormality in oral cancer from 15ng input DNA.