Analysis of pathogenesis and pathophygiological distinction of leukoplakia and development a new method of estimation of malignant alteration focused on calcium sensing receptor

为了寻找口腔黏膜白斑与CaSR基因突变的可能相关性，我们分析了与正常口腔黏膜相比，伴有/不伴有上皮角化异常的口腔黏膜白斑中CaSR的基因排列和基因突变。选取口腔黏膜白斑28例，正常口腔黏膜21例作为对照。对从上皮细胞中提取的dna进行CaSR基因突变分析。结果发现外显子7的非同义共同多态性R990G、A986S、Q11011E，未发现新的突变。不幸的是，在本研究中，我们无法揭示CaSR基因突变与白斑发生的关系。然而，据报道，CaSR在结肠癌的分化中具有复杂的作用。CaSR基因突变与口腔黏膜白斑的相关性有待进一步研究。

In the aim of finding possible correlations on CaSR gene mutation about oral mucosal leukoplakia, we analyzed gene arrangement and genetic mutation of CaSR on oral mucosal leukoplakia with/without abnormal keratosis of epithelium compared with the normal oral mucosa. 28 cases diagnosed as leukoplakia and 21 cases with normal oral mucosa (as controls) were selected. DNAs extracted from epithelial cells were analyzed about CaSR gene mutation. As a result, the nonsynonymous common polymorphisms R990G, A986S, Q11011E in exon7 were identified and another novel mutation was not observed. In this study, unfortunately, we couldn't reveal the association between genetic mutation of CaSR and development of leukoplakia. However, the CaSR had a complex role for differentiation of colon cancer was reported. Further investigation is necessary to evaluate the correlation between CaSR gene mutation and oral mucosal leukoplakia.