Analysis of pathogenesis and pathophygiological distinction of leukoplakia and development a new method of estimation of malignant alteration focused on calcium sensing receptor

分析白斑的发病机制和病理生理学特征并建立以钙敏感受体为中心的恶性改变评估新方法

• 批准号: 24659905
• 负责人: SATOMURA Kazuhito
• 金额: $ 2.33万
• 依托单位: Tsurumi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659905/
• 关键词: 口腔白板症; CaSR; 悪性化確率

In the aim of finding possible correlations on CaSR gene mutation about oral mucosal leukoplakia, we analyzed gene arrangement and genetic mutation of CaSR on oral mucosal leukoplakia with/without abnormal keratosis of epithelium compared with the normal oral mucosa. 28 cases diagnosed as leukoplakia and 21 cases with normal oral mucosa (as controls) were selected. DNAs extracted from epithelial cells were analyzed about CaSR gene mutation. As a result, the nonsynonymous common polymorphisms R990G, A986S, Q11011E in exon7 were identified and another novel mutation was not observed. In this study, unfortunately, we couldn't reveal the association between genetic mutation of CaSR and development of leukoplakia. However, the CaSR had a complex role for differentiation of colon cancer was reported. Further investigation is necessary to evaluate the correlation between CaSR gene mutation and oral mucosal leukoplakia.

为了寻找口腔粘膜白斑CaSR基因突变的可能相关性，我们以正常口腔粘膜为对照，分析了有或无上皮角化异常的口腔粘膜白斑的CaSR基因排列和基因突变。选择28例诊断为白斑的患者和21例正常口腔黏膜作为对照。对提取的上皮细胞DNA进行CaSR基因突变分析。结果发现外显子7存在R990G、A986S、Q11011E等常见的非同义多态，未发现新的突变。遗憾的是，在这项研究中，我们无法揭示CaSR基因突变与白斑发生之间的关系。然而，CaSR在结肠癌的分化中具有复杂的作用已有报道。CaSR基因突变与口腔粘膜白斑的相关性有待进一步研究。