Mechanism of transcriptional regulation from arachnoid cells to endothelial cells

蛛网膜细胞到内皮细胞的转录调控机制

• 批准号: 25670619
• 负责人: WADA Youichiro
• 金额: $ 2.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670619/
• 关键词: 脳血管障害; トランスクリプトーム; エピゲノム; くも膜

It is not fully elucidated the role of arachnoid cells in the course of subarachnoid hemorrhage. It was found that arachnoid cells could contribute to the recovery from cerebrovascular diseases. To elucidate the transcriptional regulation mechanism of arachnoid cells, we performed comprehensive transcriptional analysis followed by epigenetic analysis. We found some of histone modifications were dynamically changed, suggesting the importance of chromatin dynamics of arachnoid cells. Based on the outcome of this project, further study is now ongoing to confirm and validate the significance of these findings.

蛛网膜细胞在蛛网膜下腔出血过程中的作用尚未完全阐明。 研究发现蛛网膜细胞对脑血管病的康复有促进作用。 为了阐明蛛网膜细胞的转录调控机制，我们进行了全面的转录分析，然后进行表观遗传分析。 我们发现一些组蛋白修饰是动态变化的，提示了蛛网膜细胞染色质动力学的重要性。 根据该项目的结果，目前正在进行进一步研究，以确认和验证这些发现的重要性。

项目成果

Direct evidence for pitavastatin induced chromatin structure change in the KLF4 gene in endothelial cells.

• DOI: 10.1371/journal.pone.0096005
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Maejima T;Inoue T;Kanki Y;Kohro T;Li G;Ohta Y;Kimura H;Kobayashi M;Taguchi A;Tsutsumi S;Iwanari H;Yamamoto S;Aruga H;Dong S;Stevens JF;Poh HM;Yamamoto K;Kawamura T;Mimura I;Suehiro J;Sugiyama A;Kaneki K;Shibata H;Yoshinaka Y;Doi T;Asanuma A;Tanabe S;Tanaka T;Minami T;Hamakubo T;Sakai J;Nozaki N;Aburatani H;Nangaku M;Ruan X;Tanabe H;Ruan Y;Ihara S;Endo A;Kodama T;Wada Y
• 通讯作者: Wada Y

Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli

• DOI: 10.1016/j.molcel.2014.01.009
• 发表时间: 2014-02-06
• 期刊: MOLECULAR CELL
• 影响因子: 16
• 作者: Imamura, Katsutoshi;Imamachi, Naoto;Akimitsu, Nobuyoshi
• 通讯作者: Akimitsu, Nobuyoshi

内皮細胞において観察される転写の波が示唆する転写複合体によるクロマチン構造変化

内皮细胞中观察到的转录波表明转录复合物诱导的染色质结构变化

• 发表时间: 2013
• 作者: Imamura K;Imamachi N;Akizuki G;Kumakura M;Kawaguchi A;Nagata K;Kato A;Kawaguchi Y;Sato H;Yoneda M;Kai C;Yada T;Suzuki Y;Yamada T;Ozawa T;Kaneki K;Inoue T;Kobayashi M;Kodama T;Wada Y;Sekimizu K and Akimitsu N.;和田洋一郎
• 通讯作者: 和田洋一郎

Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.

• DOI: 10.1186/gb-2014-15-4-r63
• 发表时间: 2014-04-10
• 期刊: Genome biology
• 影响因子: 12.3
• 作者: Inoue T;Kohro T;Tanaka T;Kanki Y;Li G;Poh HM;Mimura I;Kobayashi M;Taguchi A;Maejima T;Suehiro J;Sugiyama A;Kaneki K;Aruga H;Dong S;Stevens JF;Yamamoto S;Tsutsumi S;Fujita T;Ruan X;Aburatani H;Nangaku M;Ruan Y;Kodama T;Wada Y
• 通讯作者: Wada Y