Translational Pruritus Research (PRUSEARCH): Central Information Infrastructure, Centralized Biomaterial and Machine Learning Analyses

涉及多个研究所的研究项目和协作小组对于回答复杂疾病的关键问题具有重要意义。当不同的基础研究方法满足以患者为导向的项目时，如果后者包括不同的视角、问卷和考试，情况就会变得更加复杂。这种综合办法是研究单位PRUSEARCH的基础，并通过密切合作和彻底协调取得了成功。独立的研究单位往往无法管理多学科数据、互操作性方法和生物材料加工相结合所涉及的结构和后勤。作为一个平台项目，该项目(#Z)支持其他子项目克服这些挑战，汇集专业知识和减少平行支出。我们将继续提供和管理中央信息基础设施，方法是建立一个最新的、灵活的和符合数据保护法规的数据捕获系统，连接到一个中央数据库内商定的项目空间。它包括多个临床数据的自动集成和假名、问卷回答和标准化的功能特征。此外，主页(www.prusearch.net)将扩展到可供公众访问的部分和研究小组内部的私人部分。内部部分提供对每个项目的数据库的访问。统一标准作业程序(SOP)将得到加强和更新。对所有中心招募的慢性瘙痒患者及其对照的皮肤活检的中心生物材料分析将继续并得到加强。研究单位伙伴之间的材料交换将由#Z协调，包括在不同项目中联合使用同一患者的活组织检查。此外，#Z将提供基本的材料分析，如皮肤组织病理学和材料的分子特征，以及集中的高通量信使核糖核酸测序和微生物组分析。最后，#Z将为智能数据采集提供信息基础设施，并对这些数据进行集中的生物信息学分析。将应用机器学习分析来解决数据集的复杂性。协调、统一协议和方法，在该联盟内实际处理生物材料和生物信息学，包括最先进的机器学习方法，需要一个中央项目，使该联盟能够实现其目标的附加值。

Research projects and collaborative groups that involve multiple institutes are of great importance to answer key questions of complex diseases. When different basic research approaches meet patient oriented projects and if the latter ones include different perspectives, questionnaires and examinations, it becomes even more complex. Such a comprehensive approach is the fundament of the research unit PRUSEARCH and was successful by close collaboration and thorough coordination. Stand-alone research units are often not able to manage structure and logistics involved in combining multidisciplinary data, interoperability methods and biomaterial processing. Being a platform project, this project (#Z) has supported the other subprojects to overcome these challenges, pool expertise and reducing parallel expenditures.We will continue to provide and manage a central information infrastructure by building an up-to-date flexible and data protection legislation conform data capture system connected to a consented project space within a centralized database. It includes automatic integration and pseudonymization of multiple clinical data, questionnaire responses and standardized functional characterizations. Furthermore the homepage (www.prusearch.net) will be extended both regarding the publicly accessible and the research group internal private section. The internal section provides access to the database for each project. Harmonized standard operation procedures (SOPs) will be enhanced and updated. Central biomaterial analyses for skin biopsies of all-center recruited patients with chronic pruritus and their controls will be continued and enhanced. Material exchange between research unit partners will be coordinated by #Z, including combined use of biopsies from the same patient in different projects. Furthermore, #Z will provide basic material analysis such as dermatohistopathologic and molecular characterization of material and centralized high-throughput mRNA-sequencing and microbiome analysis. Finally #Z will provide the information infrastructure for intelligent data capture and perform centralized bioinformatics analysis of these data. The complexity of the datasets will be addressed applying machine learning analyses.Coordination, harmonization of protocols and methods, actual handling of biomaterials and bioinformatics within the consortium, including state-of-the-art machine learning methods, requires a central project which will allow achievement of the additional value aimed to in this consortium.