Study of Golgi pH sensor and the mechanism for the localization

(1)As通过表达克隆鉴定了一个重要线索，以鉴定高尔基体pH传感器或其下游因子，蛋白A，其过表达纠正突变表型，如延迟转运和受损的糖基化，和蛋白B，其过表达取消蛋白A的正常化作用。（2）糖基转移酶复合物的形成受损而不是它们在高尔基体中的错误定位似乎是糖基化受损的机制之一。

（1）As an important clue to identify a Golgi pH sensor or its downstream factor, protein A whose overexpression corrects the mutant phenotypes such as delayed transport and impaired glycosylation, and protein B whose overexpression cancels the normalizing effect of protein A, were identified by expression cloning.（2）Impaired complex formation of glycosyltranferases but not their mis-localization in the Golgi seemed one of the mechanisms for impaired glycosylation.