Evolution of visual opsin gene expression through gene duplication manifested in fish and primates

鱼类和灵长类动物中通过基因复制实现视觉视蛋白基因表达的进化

• 批准号: 23657164
• 负责人: KAWAMURA Shoji
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657164/
• 关键词: オプシン; 視物質; ゼブラフィッシュ; グッピー; トランスジェニック; 発現制御領域; 霊長類; 国際情報交換; メダカ; 遺伝子重複; 発現分化; 発現制御; 小型魚類

Functional differentiation of visual opsin genes through gene duplication was investigated for fish and primates as a study model, in terms of differentiation in gene expression and absorption spectra. We contributed to understanding of retinal development by visualizing a subset of photoreceptor cells through opsin-class-specific promoters. We found that guppy LWS opsin genes have undergone genetic differentiation among wild populations. We showed that L and M opsin genes of primates have experienced homogenization between them by gene conversion in introns and hybridization, while spectral difference between L and M opsins appear to have been maintained by purifying selection.

以鱼类和灵长类动物为研究模型，从基因表达差异和吸收光谱差异两个方面研究了视蛋白基因通过基因复制的功能分化。我们通过视蛋白类特异性启动子可视化感光细胞的一个子集，为理解视网膜发育做出了贡献。我们发现孔雀鱼LWS视蛋白基因在野生种群中发生了遗传分化。我们发现，灵长类动物的L和M视蛋白基因经历了同质化，它们之间的基因转换内含子和杂交，而L和M视蛋白之间的光谱差异似乎已保持纯化选择。

项目成果

Gene conversion and purifying selection shape nucleotide variation in gibbon L/M opsin genes.

• DOI: 10.1186/1471-2148-11-312
• 发表时间: 2011-10-22
• 期刊: BMC evolutionary biology
• 影响因子: 3.4
• 作者: Hiwatashi T;Mikami A;Katsumura T;Suryobroto B;Perwitasari-Farajallah D;Malaivijitnond S;Siriaroonrat B;Oota H;Goto S;Kawamura S
• 通讯作者: Kawamura S

Comparison of gene organization and expression of blue-sensitive (SWS2) opsins among medaka fish species

青鳉鱼蓝敏（SWS2）视蛋白基因组织和表达的比较

• 发表时间: 2011
• 作者: Aso;M.;Takishima;N.;Matsumoto;Y.;Oda;S.;Mitani;H. and Kawamura;S.
• 通讯作者: S.

Evolutionary study of vertebrate color vision : from fish transgenesis to field primatology, and to human variation

脊椎动物色觉的进化研究：从鱼类转基因到野外灵长类动物学，再到人类变异

• 发表时间: 2011
• 作者: Kawamura;S.
• 通讯作者: S.

Exploration of regulatory region for differential expression of duplicated red-sensitive opsin genes in zebrafish

斑马鱼重复红色敏感视蛋白基因差异表达调控区的探索

• 发表时间: 2011
• 作者: Ashino;R.;Tsujimura;T.;Hoyoya;T. and Kawamura;S.
• 通讯作者: S.

A population genetic study on the relationship between medaka fish and the spread of wet-rice cultivation across the Japanese archipelago

青鳉鱼与日本列岛湿稻种植传播关系的群体遗传学研究

• DOI: 10.1537/ase.110525
• 发表时间: 2012
• 期刊: Anthropological Science
• 影响因子: 0.7
• 作者: Katsumura;T.;Oda;S.;Tsukamoto;K.;Sekiya;Y.;Yamashita;T.;Aso;M.;Hata;M.;Nonaka;M.;Mano;S.;Ishida;H.;Mitani;H.;Kawamura;S.;Oota;H.
• 通讯作者: H.