Clarification of metabolic mechanism of non-enzymatic glycation compounds in chickens

首先，研究了PHP-THβC对鸡胚成肌细胞蛋白质合成的交互作用。当胎牛血清浓度为10%时，PHP-THβC浓度超过4 μM时，蛋白质合成显著增加。其次，PHP-THβC的营养价值研究表明，PHP-THβC不能代替色氨酸作为蛋白质合成的前体。PHP-THβC和葡萄糖-色氨酸Amadori产物的浓度分别约为1.5μM和3.0 μM。

Firstly, the interactive effect of PHP-THβC on protein synthesis of chicken embryo myoblasts was examined. When fetal calf serum in the medium was 10%, protein synthesis was significantly increased by elevating PHP-THβC concentration more than 4 μM.Secondly, the investigation to study the nutritional value for PHP-THβC revealed that PHP-THβC could not be a precursor for protein synthesis instead of tryptophan.Thirdly, under normal condition, the concentrations of PHP-THβC and glucose-tryptophan Amadori product were approximately 1.5μM and 3.0 μM, respectively.