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STUDIES ON IGF-I SIGNAL CASCADE ON MUSCLE PROTEIN RETENTION

IGF-I 信号级联对肌肉蛋白保留的研究

基本信息

批准号：
15380194
负责人：
KITA Kazumi
金额：
$ 7.49万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

Influence of methionine and/or glycine deficiency on protein synthesis of chicken embryo myoblasts cultured in Medium 199 was examined. Protein synthesis cultured in Medium 199 in which all ingredients were solved in Dulbecco's modified phosphate buffered saline was dramatically lower than that in Medium 199 in which all ingredients were solved in water. Although methionine deficiency from Medium 199 decreased myoblast protein synthesis, glycine deficiency did not affect. There was no interaction between methionine and glycine deficiency on myoblast protein synthesis.The influence of leucine and fetal calf serum (FCS) in Medium 199 on protein synthesis and degradation of chicken embryo myoblasts was examined. Medium 199 included 0, 1, 2, 5, 10% FCS and 457 (original concentration of leucine in Medium 199), 2287, 4574μmol leucine/l. There was an interaction between leucine and FCS concentrations on muscle protein synthesis in vitro. When FCS was not included in Medium 199, myoblast prot … More ein synthesis was stimulated with an increment in leucine concentration in the medium from 2287 to 4574 μmol/l. When 1 or 2% of FCS was included in Medium 199, myoblast protein synthesis was stimulated by increasing leucine concentration in the medium from 457 to 2287 μmol/l, and at the higher level of leucine, protein synthesis was not stimulated. At more than 5% of FCS in the medium, myoblast protein synthesis at higher concentration of luecine of 4574μmol/l was significantly lower than those of lower leucine levels.No interaction between leucine and FCS concentrations on muscle protein degradation Was observed. Protein degradation of chicken embryo myoblasts was decreased by an increment of FCS concentration in the medium from 0 to 5%, and at the higher level no significant influence was observed. When leucine concentration of in the medium was increased from 457 to 2287μmol/l, protein degradation of chicken embryo myoblasts was decreased. But high concentration of leucine at 4457μmol/l did not affect protein degradation of chicken embryo myoblasts. Less

本文研究了蛋氨酸和/或甘氨酸缺乏对鸡胚成肌细胞蛋白质合成的影响。在培养基199中培养的蛋白质合成显著低于在培养基199中培养的蛋白质合成，在培养基199中，所有成分都溶解在Dulbecco改良磷酸盐缓冲盐水中，在培养基199中培养的蛋白质合成显著低于在培养基199中培养的蛋白质合成，在培养基199中，所有成分都溶解在水中。虽然从培养基199蛋氨酸缺乏减少成肌细胞蛋白质的合成，甘氨酸缺乏没有影响。蛋氨酸和甘氨酸缺乏对成肌细胞蛋白质合成无交互作用，本实验研究了199培养基中亮氨酸和胎牛血清（FCS）对成肌细胞蛋白质合成和降解的影响。培养基199包括0、1、2、5、10%FCS和457（培养基199中亮氨酸的初始浓度）、2287、4574μmol亮氨酸/l。亮氨酸和FCS浓度对体外肌肉蛋白质合成有交互作用。当培养基199中不含FCS时，成肌细胞蛋白表达增加。 ...更多信息当培养基中亮氨酸浓度从2287 μ mol/L增加到4574 μmol/L时，Ein的合成受到刺激。当培养基199中含有1%或2%的FCS时，培养基中亮氨酸浓度从457 μmol/l增加到2287 μmol/l可刺激成肌细胞蛋白质合成，但在较高水平的亮氨酸下，蛋白质合成不受刺激。当FCS浓度大于5%时，高浓度亮氨酸（4574μmol/l）的蛋白质合成量显著低于低浓度亮氨酸的蛋白质合成量，但亮氨酸和FCS浓度对蛋白质降解无交互作用。培养液中FCS浓度从0增加到5%时，鸡胚成肌细胞的蛋白质降解降低，在较高浓度时没有观察到显著影响。当培养液中亮氨酸浓度从457 μmol/L增加到2287μmol/L时，鸡胚成肌细胞蛋白质降解减少。但高浓度亮氨酸（4457μmol/L）对鸡胚成肌细胞蛋白质降解无影响。少