Accurate estimation of amino acid requirement using the potency of specific amino acids to stimulate muscle protein synthesis

在单一氨基酸缺乏的培养液中，发现缬氨酸和蛋氨酸促进鸡胚成肌细胞蛋白质合成。缬氨酸刺激蛋白质合成，提高胰岛素样生长因子1型受体的基因表达。然而，日粮中添加缬氨酸并没有改善鸡的生长性能。在低蛋白日粮中同时添加蛋氨酸和甘氨酸可提高肉仔鸡的饲料利用率。

It was found that valine and methionine stimulated protein synthesis when chicken embryo myoblasts were incubated in single amino acid deficient medium. Valine stimulated protein synthesis with elevation of gene expression of insulin-like growth factor type-1 receptor. However, dietary valine supplementation did not improve growth performance of chickens. Supplementation of both methionine and glycine in a low-protein diet improved feed efficiency of broilers.