Accurate estimation of amino acid requirement using the potency of specific amino acids to stimulate muscle protein synthesis

结果表明，缬氨酸和蛋氨酸能刺激鸡胚成肌细胞在单氨基酸缺乏培养基中合成蛋白质。缬氨酸通过提高胰岛素样生长因子1型受体的基因表达来刺激蛋白质合成。然而，饲粮中添加缬氨酸并没有提高鸡的生长性能。低蛋白饲粮中同时添加蛋氨酸和甘氨酸可提高肉鸡的饲料效率。

It was found that valine and methionine stimulated protein synthesis when chicken embryo myoblasts were incubated in single amino acid deficient medium. Valine stimulated protein synthesis with elevation of gene expression of insulin-like growth factor type-1 receptor. However, dietary valine supplementation did not improve growth performance of chickens. Supplementation of both methionine and glycine in a low-protein diet improved feed efficiency of broilers.