Synthesis of Functional Polysaccharides and Their Derivations to Biologically Active Polymer Materials

功能多糖的合成及其生物活性高分子材料的衍生物

• 批准号: 58850185
• 负责人: URYU Toshiyuki
• 金额: $ 5.12万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1983
• 资助国家: 日本
• 起止时间: 1983 至 1985
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-58850185/
• 关键词: Synthetic polysaccharide; Poly-ribose; Synthetic branched dextran; Sulfated poly-ribose; Anticoagulant activity; 抗原-抗体反応

The purpose of this investigation is to synthesize biologically active polysaccharides by the conversion of polysaccharide derivatives which are prepared by ring-opening polymerizations of anhydro sugars obtained from natural carbohydrates.1. Synthesis of Biologically Active Polymer Materials from Ribose Polymers and Copolymers. Although a sulfated (1->5)- <alpha> -D-ribofuranan which was prepared by ring-opening polymerization of a 1,4-anhydro-ribose derivative showed a high anticoagulant activity, a non-stereoregular polymer with a mixed structure consisting of (1->5)- <alpha> -D-ribofuranosidic and (1->4)- <beta> -D-ribopyranosidic units was sulfated to give a heparinoid with a low activity. Next, a stereoregular copolysaccharide (1->5)- <alpha> -riboxylan which is composed of 34% ribofuranosidic and 66% xylofuranosidic units was obtained by copolymerization of the two monomers. The sulfated (1->5)- <alpha> -riboxylan showed a lower anticoagulant activity than that of the individual … More homopolymers, but it was higher than that of the non-stereoregular poly-ribose. Antigen-antibody reaction of polysaccharides was examined by reacting ribopyranan, ribofuranan containing a small fraction of ribopyranose units, and ribofuranan with an antibody made by the injection of (1->4)- <beta> -Dribopyranan. The reaction between the ribopyranan and the antibody well took place. However, no reaction between the ribofuranan and the antibody occurred. And, the reaction to a small extent occurred in the case of the ribofuranan containing a small fraction of ribopyranose units, In addition, the ability for the ribofuranan to produce an antibody was too weak to give no antibody in the rabbit.2. Synthesis of Branched Polysaccharides and Its Biological Function. A dextran with <alpha> -D-mannopyranosyl branches at C-3 position was synthesized. from polymerization of tert-butyldimethylsilylated 1,6-anhydro- <beta> -D-glucopyranose and subsequent branching reaction. The mannose-branched dextran reacted with an anti-natural-dextran to a almost equal extent to a glucose-branched. Less

本研究的目的是将天然碳水化合物中的无水糖开环聚合得到的多糖衍生物转化为具有生物活性的多糖。由核糖聚合物和共聚物合成生物活性聚合物材料。尽管通过1，4-无氢核糖衍生物开环聚合制备的(1-&gt；5)-&lt；α&gt；-D-核呋喃具有较高的抗凝血活性，但由(1-&gt；5)-&lt；α&gt；-D-呋喃核苷和(1-&gt；4)-lt；β&gt；-D-核吡喃糖苷单元组成的混合结构的非立体规则聚合物被硫酸盐化，得到活性较低的类肝素。然后，通过两个单体的共聚反应，得到了一种立体正规共聚糖(1-&gt；5)-lt；α-gt；-核木聚糖，它由34%的呋喃核糖和66%的木呋喃糖基组成。硫化(1->5)-α-核木聚糖的抗凝血活性低于单独的…。均聚物较多，但高于非立体规则多聚核糖。通过注射(1&gt；4)-&lt；β&gt；-Dribopyranan制备的抗体与核吡喃、含有少量核糖单元的核呋喃和核呋喃反应，检测了多糖的抗原-抗体反应。核吡喃与抗体发生了良好的反应。然而，核呋喃与抗体之间没有发生反应。而且，在含有少量核吡喃单元的核呋喃的情况下，反应发生的程度很小，此外，核呋喃产生抗体的能力太弱，不能在兔体内产生抗体。支链多糖的合成及其生物学功能。合成了C-3位含&lt；α&gt；-D-吡喃甘露糖基支链的右旋糖苷。由叔丁基二甲基硅烷化的1，6-脱水-1，6-β-D-吡喃葡萄糖聚合和随后的支化反应而得。甘露糖支链的葡聚糖与反天然葡聚糖的反应程度几乎等同于葡萄糖支链的反应。较少

项目成果

J.Biochemistry. 99-3. (1986)

J.生物化学。

J. Biochemistry. 99-1. (1986)

J.生物化学。

J.Biochemistry. 99-1. (1986)

J.生物化学。

Macromolecules. 19-1. (1986)

高分子。

Macromolecules. 18-4. (1985)

高分子。